Anti-GCK Antibody (CAB6293)
- SKU:
- CAB6293
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GCK Antibody |
| Antibody SKU: | CAB6293 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCK (NP_000153.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCK (NP_000153.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLDD RARM EAAK KEKV EQIL AEFQ LQEE DLKK VMRR MQKE MDRG LRLE THEE ASVK MLPT YVRS TPEG SEVG DFLS LDLG GTNF RVML VKVG EGEE GQWS VKTK HQMY SIPE DAMT GTAE MLFD YISE CISD FLDK HQMK HKKL PLGF TFSF PVRH EDID KGIL LNWT KGFK ASGA EGNN VVGL LRDA IKRR GDFE MDVV AMVN DTVA TMIS CYYE DHQC EVGM IVGT GCNA CYME EMQN VELV EGDE GRMC VNTE WGAF GDSG ELDE FLLE YDRL VDES SANP GQQL YEKL IGGK YMGE |
| Gene ID: | 2645 |
| Uniprot: | P35557 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 52kDa |
| Observed MW: | 52kDa |
| Synonyms: | GCK, FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2 |
| Background: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). |
| UniProt Code: | P35557 |
| NCBI GenInfo Identifier: | 547696 |
| NCBI Gene ID: | 2645 |
| NCBI Accession: | P35557.1 |
| UniProt Secondary Accession: | P35557,Q05810, A4D2J2, A4D2J3, |
| UniProt Related Accession: | P35557 |
| Molecular Weight: | 465 |
| NCBI Full Name: | Glucokinase |
| NCBI Synonym Full Names: | glucokinase (hexokinase 4) |
| NCBI Official Symbol: | GCK |
| NCBI Official Synonym Symbols: | GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3 |
| NCBI Protein Information: | glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase |
| UniProt Protein Name: | Glucokinase |
| UniProt Synonym Protein Names: | Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D |
| UniProt Gene Name: | GCK |
| UniProt Entry Name: | HXK4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GCK antibody (CAB6293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U2OS cells using GCK antibody (CAB6293). Blue: DAPI for nuclear staining. |
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