Anti-GCK Antibody (CAB15059)
- SKU:
- CAB15059
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GCK Antibody |
| Antibody SKU: | CAB15059 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human GCK (NP_000153.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, mouse liver, rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human GCK (NP_000153.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RMCV NTEW GAFG DSGE LDEF LLEY DRLV DESS ANPG QQLY EKLI GGKY MGEL VRLV LLRL VDEN LLFH GEAS EQLR TRGA FETR FVSQ VESD TGDR KQIY NILS TLGL RPST TDCD IVRR ACES VSTR AAHM CSAG LAGV INRM RESR SEDV MRIT VGVD GSVY KLHP SFKE RFHA SVRR LTPS CEIT FIES EEGS GRGA ALVS AVAC KKAC MLGQ |
| Gene ID: | 2645 |
| Uniprot: | P35557 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 52kDa |
| Observed MW: | 80/55kDa |
| Synonyms: | GCK, FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2 |
| Background: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). |
| UniProt Code: | P35557 |
| NCBI GenInfo Identifier: | 547696 |
| NCBI Gene ID: | 2645 |
| NCBI Accession: | P35557.1 |
| UniProt Secondary Accession: | P35557,Q05810, A4D2J2, A4D2J3, |
| UniProt Related Accession: | P35557 |
| Molecular Weight: | 465 |
| NCBI Full Name: | Glucokinase |
| NCBI Synonym Full Names: | glucokinase (hexokinase 4) |
| NCBI Official Symbol: | GCK |
| NCBI Official Synonym Symbols: | GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3 |
| NCBI Protein Information: | glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase |
| UniProt Protein Name: | Glucokinase |
| UniProt Synonym Protein Names: | Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D |
| UniProt Gene Name: | GCK |
| UniProt Entry Name: | HXK4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GCK antibody (CAB15059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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