Anti-GART Antibody (CAB3876)
- SKU:
- CAB3876
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GART Antibody |
| Antibody SKU: | CAB3876 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human GART (NP_000810.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, HepG2, HeLa, K-562, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human GART (NP_000810.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAR VLII GSGG REHT LAWK LAQS HHVK QVLV APGN AGTA CSEK ISNT AISI SDHT ALAQ FCKE KKIE FVVV GPEA PLAA GIVG NLRS AGVQ CFGP TAEA AQLE SSKR FAKE FMDR HGIP TAQW KAFT KPEE ACSF ILSA DFPA LVVK ASGL AAGK GVIV AKSK EEAC KAVQ EIMQ EKAF GAAG ETIV IEEL LDGE EVSC LCFT DGKT VAPM PPAQ DHKR LLEG DGGP NTGG MGAY CPAP QVSN DLLL KIKD TVLQ RTVD GMQQ EGTP YTGI LYAG IMLT KNGP KVLE FNCR FGDP ECQV ILPL LKSD LYEV IQST LDGL |
| Gene ID: | 2618 |
| Uniprot: | P22102 |
| Cellular Location: | |
| Calculated MW: | 46kDa/107kDa |
| Observed MW: | 120kDa |
| Synonyms: | GART, AIRS, GARS, GARTF, PAIS, PGFT, PRGS |
| Background: | The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Methyltransferase; Ligase; EC 6.3.3.1; Nucleotide Metabolism - purine; EC 2.1.2.2; EC 6.3.4.13; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Enzyme, cellular metabolism Chromosomal Location of Human Ortholog: 21q22.11 Cellular Component: cytosol Molecular Function:methyltransferase activity; metal ion binding; phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity; ATP binding Biological Process: methylation; response to organic substance; purine ribonucleoside monophosphate biosynthetic process; glycine metabolic process; tetrahydrofolate biosynthetic process; response to inorganic substance; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22102 |
| NCBI GenInfo Identifier: | 131616 |
| NCBI Gene ID: | 2618 |
| NCBI Accession: | P22102.1 |
| UniProt Related Accession: | P22102 |
| Molecular Weight: | 120kDa |
| NCBI Full Name: | Trifunctional purine biosynthetic protein adenosine-3 |
| NCBI Synonym Full Names: | phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase |
| NCBI Official Symbol: | GART |
| NCBI Official Synonym Symbols: | AIRS; GARS; PAIS; PGFT; PRGS; GARTF |
| NCBI Protein Information: | trifunctional purine biosynthetic protein adenosine-3 |
| UniProt Protein Name: | Trifunctional purine biosynthetic protein adenosine-3 |
| UniProt Synonym Protein Names: | Glycinamide ribonucleotide synthetase; GARS; Phosphoribosylglycinamide synthetase |
| UniProt Gene Name: | GART |
| UniProt Entry Name: | PUR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GART antibody (CAB3876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Human GART (GAR transformylase) ELISA Kit (HUFI04170)
Anti-WBP1 Antibody (CAB13212)
Anti-STAB2 Antibody (CAB10074)
