Anti-GALT Antibody (CAB19903)[KO Validated]

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SKU:
CAB19903
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-GALT Antibody [KO Validated]
Antibody SKU:CAB19903
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human GALT.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human GALT.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2592
Uniprot:P07902
Cellular Location:
Calculated MW:31kDa/43kDa
Observed MW:43KDa
Anti-GALT Antibody [KO Validated] (CAB19903)Western blot analysis of extracts from normal (control) and GALT knockout (KO) HeLa cells, using GALT antibody (CAB19903) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:GALT
Background:Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.
UniProt Protein Details:

Protein type:EC 2.7.7.12; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cytosol; Golgi apparatus

Molecular Function:protein binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity

Biological Process: galactose catabolic process; galactose metabolic process

Disease: Galactosemia

NCBI Summary:Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P07902
NCBI GenInfo Identifier:3183522
NCBI Gene ID:2592
NCBI Accession:P07902.3
UniProt Secondary Accession:P07902,Q14355, Q14356, Q14357, Q14358, Q14359, Q14360 Q14361, Q14363, Q14364, B4E097, E7ET32,
UniProt Related Accession:P07902
Molecular Weight:31,107 Da
NCBI Full Name:Galactose-1-phosphate uridylyltransferase
NCBI Synonym Full Names:galactose-1-phosphate uridylyltransferase
NCBI Official Symbol:GALT  
NCBI Protein Information:galactose-1-phosphate uridylyltransferase
UniProt Protein Name:Galactose-1-phosphate uridylyltransferase
UniProt Synonym Protein Names:UDP-glucose--hexose-1-phosphate uridylyltransferase
Protein Family:Gallate transporter
UniProt Gene Name:GALT  
UniProt Entry Name:GALT_HUMAN

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