Anti-GABARAP 항체 (CAB5616)

Antibody Name:	Anti-GABARAP Antibody
Antibody SKU:	CAB5616
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAP (NP_009209.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, SH-SY5Y, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAP (NP_009209.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKFV YKEE HPFE KRRS EGEK IRKK YPDR VPVI VEKA PKAR IGDL DKKK YLVP SDLT VGQF YFLI RKRI HLRA EDAL FFFV NNVI PPTS ATMG QLYQ EHHE EDFF LYIA YSDE SVYG L
Gene ID:	11337
Uniprot:	O95166
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus membrane, autophagosome, cytoskeleton
Calculated MW:	13kDa
Observed MW:	16kDa

Synonyms:

GABARAP, ATG8A, GABARAP-a, MM46

Background:

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

UniProt Protein Function:	GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.
UniProt Protein Details:	Protein type:Autophagy; Adaptor/scaffold; Microtubule-binding; Ubiquitin-like modifier; Vesicle Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; axoneme; cytoplasmic vesicle Molecular Function:protein binding; microtubule binding; GABA receptor binding; beta-tubulin binding Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; autophagic vacuole formation; protein targeting
NCBI Summary:	Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]
UniProt Code:	O95166
NCBI GenInfo Identifier:	44887916
NCBI Gene ID:	11337
NCBI Accession:	O95166.1
UniProt Related Accession:	O95166
Molecular Weight:	117
NCBI Full Name:	Gamma-aminobutyric acid receptor-associated protein
NCBI Synonym Full Names:	GABA(A) receptor-associated protein
NCBI Official Symbol:	GABARAP
NCBI Official Synonym Symbols:	MM46; ATG8A; GABARAP-a
NCBI Protein Information:	gamma-aminobutyric acid receptor-associated protein
UniProt Protein Name:	Gamma-aminobutyric acid receptor-associated protein
UniProt Synonym Protein Names:	GABA(A) receptor-associated protein; MM46
Protein Family:	Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:	GABARAP
UniProt Entry Name:	GBRAP_HUMAN

	Western blot analysis of extracts of various cell lines, using GABARAP antibody (CAB5616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody (CAB5616) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody (CAB5616) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using GABARAP antibody (CAB5616) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using GABARAP antibody (CAB5616). Blue: DAPI for nuclear staining.

Antibodies

Anti-GABARAP Antibody (CAB12568)

Anti-GABARAP Antibody (CAB13855)

Anti-GABARAP Antibody (CAB4335)

GABARAP Antibody (PACO09377)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)