Anti-GABARAP Antibody (CAB12568)

(No reviews yet) Write a Review
SKU:
CAB12568
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-GABARAP Antibody
Antibody SKU:CAB12568
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Jurkat, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKFV YKEE HPFE KRRS EGEK IRKK YPDR VPVI VEKA PKAR IGDL DKKK YLVP SDLT VGQF YFLI RKRI HLRA EDAL FFFV NNVI PPTS ATMG QLYQ EHHE
Gene ID:11337
Uniprot:O95166
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus membrane, autophagosome, cytoskeleton
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:GABARAP, ATG8A, GABARAP-a, MM46
Background:Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
UniProt Protein Function:GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.
UniProt Protein Details:

Protein type:Autophagy; Adaptor/scaffold; Microtubule-binding; Ubiquitin-like modifier; Vesicle

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; axoneme; cytoplasmic vesicle

Molecular Function:protein binding; microtubule binding; GABA receptor binding; beta-tubulin binding

Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; autophagic vacuole formation; protein targeting

NCBI Summary:Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]
UniProt Code:O95166
NCBI GenInfo Identifier:44887916
NCBI Gene ID:11337
NCBI Accession:O95166.1
UniProt Related Accession:O95166
Molecular Weight:117
NCBI Full Name:Gamma-aminobutyric acid receptor-associated protein
NCBI Synonym Full Names:GABA(A) receptor-associated protein
NCBI Official Symbol:GABARAP  
NCBI Official Synonym Symbols:MM46; ATG8A; GABARAP-a  
NCBI Protein Information:gamma-aminobutyric acid receptor-associated protein
UniProt Protein Name:Gamma-aminobutyric acid receptor-associated protein
UniProt Synonym Protein Names:GABA(A) receptor-associated protein; MM46
Protein Family:Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:GABARAP  
UniProt Entry Name:GBRAP_HUMAN
Anti-GABARAP Antibody (CAB12568)Western blot analysis of extracts of various cell lines, using GABARAP antibody (CAB12568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-GABARAP Antibody (CAB12568)Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody (CAB12568) at dilution of 1:100 (40x lens).
Anti-GABARAP Antibody (CAB12568)Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody (CAB12568) at dilution of 1:100 (40x lens).
Anti-GABARAP Antibody (CAB12568)Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody (CAB12568) at dilution of 1:100 (40x lens).

0 Reviews

View AllClose

Related Products