Anti-FZR1 Antibody (CAB5550)

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SKU:
CAB5550
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-FZR1 Antibody
Antibody SKU:CAB5550
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse heart, Mouse spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DLSV EGDS VTSV GWSE RGNL VAVG THKG FVQI WDAA AGKK LSML EGHT ARVG ALAW NAEQ LSSG SRDR MILQ RDIR TPPL QSER RLQG HRQE VCGL KWST DHQL LASG GNDN KLLV WNHS SLSP VQQY TEHL AAVK AIAW SPHQ HGLL ASGG GTAD RCIR FWNT LTGQ PLQC IDTG SQVC NLAW SKHA NELV STHG YSQN QILV WKYP SLTQ VAKL TGHS YRVL YLAM SPDG EAIV TGAG DETL RFWN VFSK TRST KESV SVLN LFTR IR
Gene ID:51343
Uniprot:Q9UM11
Cellular Location:Cytoplasm, Nucleus
Calculated MW:44kDa/54kDa/55kDa
Observed MW:50kDa
Synonyms:FZR1, CDC20C, CDH1, FZR, FZR2, HCDH, HCDH1
Background:
UniProt Protein Function:FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: anaphase-promoting complex; cytosol; nuclear membrane; nucleoplasm

Molecular Function:protein binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition DNA damage checkpoint; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle

UniProt Code:Q9UM11
NCBI GenInfo Identifier:37537753
NCBI Gene ID:51343
NCBI Accession:Q9UM11.2
UniProt Secondary Accession:Q9UM11,O75869, Q86U66, Q96NW8, Q9UI96, Q9ULH8, Q9UM10 Q9UNQ1, Q9Y2T8,
UniProt Related Accession:Q9UM11
Molecular Weight:44,874 Da
NCBI Full Name:Fizzy-related protein homolog
NCBI Synonym Full Names:fizzy/cell division cycle 20 related 1
NCBI Official Symbol:FZR1  
NCBI Official Synonym Symbols:FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C  
NCBI Protein Information:fizzy-related protein homolog
UniProt Protein Name:Fizzy-related protein homolog
UniProt Synonym Protein Names:CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1
Protein Family:Fizzy-related protein
UniProt Gene Name:FZR1  
UniProt Entry Name:FZR_HUMAN
Anti-FZR1 Antibody (CAB5550)Western blot analysis of extracts of various cell lines, using FZR1 antibody (CAB5550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s.
Anti-FZR1 Antibody (CAB5550)Immunofluorescence analysis of HeLa cells using FZR1 antibody (CAB5550). Blue: DAPI for nuclear staining.

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