Anti-FZD1 Antibody (CAB10809)
- SKU:
- CAB10809
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-FZD1 Antibody |
| Antibody SKU: | CAB10809 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-320 of human FZD1 (NP_003496.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse eye, Mouse kidney, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-320 of human FZD1 (NP_003496.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PDTL KCEK FPVH GAGE LCVG QNTS DKGT PTPS LLPE FWTS NPQH GGGG HRGG FPGG AGAS ERGK FSCP RALK VPSY LNYH FLGE KDCG APCE PTKV YGLM YFGP EELR FSR |
| Gene ID: | 8321 |
| Uniprot: | Q9UP38 |
| Cellular Location: | Cell membrane, Membrane, Multi-pass membrane protein |
| Calculated MW: | 71kDa |
| Observed MW: | 90kDa |
| Synonyms: | FZD1 |
| Background: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues. |
| UniProt Protein Function: | FZD1: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Belongs to the G-protein coupled receptor Fz/Smo family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral; Receptor, GPCR Chromosomal Location of Human Ortholog: 7q21 Cellular Component: cell surface; focal adhesion; integral to membrane; plasma membrane Molecular Function:frizzled binding; G-protein coupled receptor activity; PDZ domain binding; protein binding; receptor binding; Wnt receptor activity; Wnt-protein binding Biological Process: cell-cell signaling; neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; response to drug; synaptogenesis; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UP38 |
| NCBI GenInfo Identifier: | 92058705 |
| NCBI Gene ID: | 8321 |
| NCBI Accession: | Q9UP38.2 |
| UniProt Secondary Accession: | Q9UP38,O94815, Q549T8, A4D1E8, |
| UniProt Related Accession: | Q9UP38 |
| Molecular Weight: | 71,158 Da |
| NCBI Full Name: | Frizzled-1 |
| NCBI Synonym Full Names: | frizzled class receptor 1 |
| NCBI Official Symbol: | FZD1 |
| NCBI Protein Information: | frizzled-1 |
| UniProt Protein Name: | Frizzled-1 |
| UniProt Synonym Protein Names: | FzE1 |
| Protein Family: | Frizzled |
| UniProt Gene Name: | FZD1 |
| UniProt Entry Name: | FZD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FZD1 antibody (CAB10809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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