Anti-FXR1 Antibody (CAB5942)
- SKU:
- CAB5942
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-FXR1 Antibody |
| Antibody SKU: | CAB5942 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FXR1 (NP_005078.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, MCF7, Mouse brain, Mouse kidney, Mouse heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FXR1 (NP_005078.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VTAI ELDE DTGT FRIY GESA DAVK KARG FLEF VEDF IQVP RNLV GKVI GKNG KVIQ EIVD KSGV VRVR IEGD NENK LPRE DGMV PFVF VGTK ESIG NVQV L |
| Gene ID: | 8087 |
| Uniprot: | P51114 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 59kDa/60kDa/69kDa |
| Observed MW: | 68kDa |
| Synonyms: | FXR1, FXR1P |
| Background: | The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FXR1: RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. Interacts with FMR1 and FXR2. Interacts with TDRD3, and with CYFIP2 but not with CYFIP1. Expressed in all tissues examined including heart, brain, kidney and testis. Belongs to the FMR1 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 3q28 Cellular Component: polysome; costamere; membrane; cytoplasm; nucleolus Molecular Function:mRNA binding; mRNA 3'-UTR binding; RNA binding Biological Process: muscle development; apoptosis; negative regulation of translation; cell differentiation |
| NCBI Summary: | The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P51114 |
| NCBI GenInfo Identifier: | 189047132 |
| NCBI Gene ID: | 8087 |
| NCBI Accession: | P51114.3 |
| UniProt Secondary Accession: | P51114,Q7Z450, Q8N6R8, A8K9B8, |
| UniProt Related Accession: | P51114 |
| Molecular Weight: | 621 |
| NCBI Full Name: | Fragile X mental retardation syndrome-related protein 1 |
| NCBI Synonym Full Names: | fragile X mental retardation, autosomal homolog 1 |
| NCBI Official Symbol: | FXR1 |
| NCBI Official Synonym Symbols: | FXR1P |
| NCBI Protein Information: | fragile X mental retardation syndrome-related protein 1; hFXR1p |
| UniProt Protein Name: | Fragile X mental retardation syndrome-related protein 1 |
| Protein Family: | Fragile X mental retardation syndrome-related protein |
| UniProt Gene Name: | FXR1 |
| UniProt Entry Name: | FXR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FXR1 antibody (CAB5942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using FXR1 antibody (CAB5942) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using FXR1 antibody (CAB5942) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using FXR1 antibody (CAB5942) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using FXR1 antibody (CAB5942) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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