Anti-FTO Antibody (CAB1438)
- SKU:
- CAB1438
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-FTO Antibody |
| Antibody SKU: | CAB1438 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-505 of human FTO (NP_001073901.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, SH-SY5Y, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-505 of human FTO (NP_001073901.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AVLH EVKR EGLP VEQR NEIL TAIL ASLT ARQN LRRE WHAR CQSR IART LPAD QKPE CRPY WEKD DASM PLPF DLTD IVSE LRGQ LLEA KP |
| Gene ID: | 79068 |
| Uniprot: | Q9C0B1 |
| Cellular Location: | Nucleus, Nucleus speckle |
| Calculated MW: | 6kDa/12kDa/14kDa/58kDa |
| Observed MW: | 60KDa |
| Synonyms: | FTO, ALKBH9, BMIQ14, GDFD |
| Background: | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. |
| UniProt Protein Function: | FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.- Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: nuclear speck; nucleus Molecular Function:ferrous iron binding; DNA-N1-methyladenine dioxygenase activity Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death |
| NCBI Summary: | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
| UniProt Code: | Q9C0B1 |
| NCBI GenInfo Identifier: | 148841515 |
| NCBI Gene ID: | 79068 |
| NCBI Accession: | Q9C0B1.3 |
| UniProt Secondary Accession: | Q9C0B1,Q0P676, Q7Z785, A2RUH1, B2RNS0, |
| UniProt Related Accession: | Q9C0B1 |
| Molecular Weight: | 12,218 Da |
| NCBI Full Name: | Alpha-ketoglutarate-dependent dioxygenase FTO |
| NCBI Synonym Full Names: | fat mass and obesity associated |
| NCBI Official Symbol: | FTO |
| NCBI Official Synonym Symbols: | ALKBH9 |
| NCBI Protein Information: | alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; AlkB homolog 9; fat mass and obesity-associated protein |
| UniProt Protein Name: | Alpha-ketoglutarate-dependent dioxygenase FTO |
| UniProt Synonym Protein Names: | Fat mass and obesity-associated protein |
| Protein Family: | Alpha-ketoglutarate-dependent dioxygenase |
| UniProt Gene Name: | FTO |
| UniProt Entry Name: | FTO_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FTO antibody (CAB1438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of extracts of Rat brain, using FTO antibody (CAB1438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat testis using FTO antibody (CAB1438) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using FTO antibody (CAB1438) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug FTO antibody (CAB1438). Western blot was performed from the immunoprecipitate using FTO antibody (CAB1438) at a dilition of 1:1000. |
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