Anti-FOXP2 항체 (CAB5677)

Antibody Name:	Anti-FOXP2 Antibody
Antibody SKU:	CAB5677
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human FOXP2 (NP_683696.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, HeLa, SH-SY5Y, Raji, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human FOXP2 (NP_683696.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PKPS PKPL NLVS SVTM SKNM LETS PQSL PQTP TTPT APVT PITQ GPSV ITPA SVPN VGAI RRRH SDKY NIPM SSEI APNY EFYK NADV RPPF TYAT LIRQ AIME SSDR QLTL NEIY SWFT RTFA YFRR NAAT WKNA VRHN LSLH KCFV RVEN VKGA VWTV DEVE YQKR RSQK ITGS PTLV KNIP TSLG YGAA LNAS LQAA LAES SLPL LSNP GLIN NASS GLLQ AVHE DLNG SLDH IDSN GNSS PGCS PQPH IHSI HVKE EPVI AEDE DCPM SLVT TANH SPEL EDDR EIEE EPLS EDLE
Gene ID:	93986
Uniprot:	O15409
Cellular Location:	Nucleus
Calculated MW:	9-18kDa/40- 48kDa/70- 82kDa
Observed MW:	80kDa

Synonyms:

FOXP2, CAGH44, SPCH1, TNRC10

Background:

This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.

UniProt Protein Function:	FOXP2: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. Forms homodimers and heterodimers with FOXP1 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; DNA-binding Chromosomal Location of Human Ortholog: 7q31 Cellular Component: nucleus Molecular Function:protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity Biological Process: skeletal muscle development; caudate nucleus development; camera-type eye development; transcription, DNA-dependent; righting reflex; putamen development; negative regulation of transcription from RNA polymerase II promoter; vocal learning; post-embryonic development; smooth muscle development; positive regulation of mesenchymal cell proliferation; cerebellum development; cerebral cortex development; negative regulation of transcription, DNA-dependent; alveolus development; growth Disease: Speech-language Disorder 1
NCBI Summary:	This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
UniProt Code:	O15409
NCBI GenInfo Identifier:	17432967
NCBI Gene ID:	93986
NCBI Accession:	O15409.2
UniProt Secondary Accession:	O15409,Q6ZND1, Q75MJ3, Q8IZE0, Q8N0W2, Q8N6B7, Q8N6B8 Q8NFQ1, A0AUV6, A4D0U8, A6NNW4, B4DLD9,
UniProt Related Accession:	O15409
Molecular Weight:	715
NCBI Full Name:	Forkhead box protein P2
NCBI Synonym Full Names:	forkhead box P2
NCBI Official Symbol:	FOXP2
NCBI Official Synonym Symbols:	SPCH1; CAGH44; TNRC10
NCBI Protein Information:	forkhead box protein P2; CAG repeat protein 44; trinucleotide repeat containing 10; forkhead/winged-helix transcription factor; trinucleotide repeat-containing gene 10 protein
UniProt Protein Name:	Forkhead box protein P2
UniProt Synonym Protein Names:	CAG repeat protein 44; Trinucleotide repeat-containing gene 10 protein
Protein Family:	Forkhead box protein
UniProt Gene Name:	FOXP2
UniProt Entry Name:	FOXP2_HUMAN

Western blot analysis of extracts of various cell lines, using FOXP2 antibody (CAB5677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

FOXP2 Antibody (PACO09342)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)