Anti-FoxO3a Antibody (CAB0102)
- SKU:
- CAB0102
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FoxO3a Antibody |
| Antibody SKU: | CAB0102 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, 293T, PC-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ALPP SQPS PPGG LMQR GSSF PYTA KSSG LGSP TGSF NSTV FGPS SLNS LRQS PMQT IQEN RPAT FSSV SHYG NQTL QDLL ASDS LSHS DVMM TQSD PLMS QAST AVSA QNAR RNVM LRND PMMS FAAQ PTQG SLVN QNLL HHQH QTQG ALGG SRAL SNSV SNMG LSDS SSLG SAKH QQQS PASQ SMQT LSDS LSGS SLYS ASAN LPVM GHDK FPSD LDLD MFNG SLEC DMES IIRS ELMD ADGL DFNF DSLI STQN VVGL NVGN FTGA KQAS SQSW VPG |
| Gene ID: | 56484 |
| Uniprot: | Q9WVH4 |
| Cellular Location: | Cytoplasm, Nucleus, cytosol |
| Calculated MW: | 71kDa |
| Observed MW: | 80-90KDa |
| Synonyms: | FOXO3, AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A |
| Background: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. |
| UniProt Protein Function: | FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. |
| UniProt Protein Details: | Protein type:Autophagy; DNA-binding; Oncoprotein; Transcription factor Chromosomal Location of Human Ortholog: 10 B2|10 22.79 cM Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus Molecular Function:beta-catenin binding; chromatin DNA binding; DNA binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity Biological Process: aging; antral ovarian follicle growth; brain morphogenesis; DNA damage response, signal transduction by p53 class mediator; glucose homeostasis; initiation of primordial ovarian follicle growth; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; oocyte maturation; ovulation from ovarian follicle; positive regulation of apoptosis; positive regulation of erythrocyte differentiation; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of translation; tumor necrosis factor-mediated signaling pathway |
| UniProt Code: | Q9WVH4 |
| NCBI GenInfo Identifier: | 81917900 |
| NCBI Gene ID: | 56484 |
| NCBI Accession: | Q9WVH4.1 |
| UniProt Secondary Accession: | Q9WVH4,Q05CZ4, D3Z6Y6, |
| UniProt Related Accession: | Q9WVH4 |
| Molecular Weight: | 71,064 Da |
| NCBI Full Name: | Forkhead box protein O3 |
| NCBI Synonym Full Names: | forkhead box O3 |
| NCBI Official Symbol: | Foxo3 |
| NCBI Official Synonym Symbols: | Fkhr2; C76856; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik |
| NCBI Protein Information: | forkhead box protein O3 |
| UniProt Protein Name: | Forkhead box protein O3 |
| Protein Family: | Forkhead box protein |
| UniProt Gene Name: | Foxo3 |
 | Western blot analysis of extracts of various cell lines, using FoxO3a antibody (CAB0102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Human colon using FoxO3a Rabbit pAb (CAB0102) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using FoxO3a Rabbit pAb (CAB0102) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of C6 cells using FoxO3a Rabbit pAb (CAB0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using FoxO3a Rabbit pAb (CAB0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
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