Anti-FKBP8 Antibody (CAB7085)

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SKU:
CAB7085
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-FKBP8 Antibody
Antibody SKU:CAB7085
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human FKBP8 (NP_036313.3).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, THP-1, BT-474, HeLa, HL-60, Mouse liver, Mouse brain, Mouse eye, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human FKBP8 (NP_036313.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QPPA EEAE QPGA LARE FLAA MEPE PAPA PAPE EWLD ILGN GLLR KKTL VPGP PGSS RPVK GQVV TVHL QTSL ENGT RVQE EPEL VFTL GDCD VIQA LDLS VPLM DVGE TAMV TADS KYCY GPQG SRSP YIPP HAAL CLEV TLKT AVDG PDLE MLTG QERV ALAN RKRE CGNA HYQR ADFV LAAN SYDL AIKA ITSS AKVD MTFE EEAQ LLQ
Gene ID:23770
Uniprot:Q14318
Cellular Location:Cytoplasmic side, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:27kDa/44kDa
Observed MW:55kDa
Synonyms:FKBP8, FKBP38, FKBPr38
Background:The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function.
UniProt Protein Function:FKBP8: Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Chaperone; Isomerase; Mitochondrial; EC 5.2.1.8; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: endoplasmic reticulum membrane; membrane; mitochondrial membrane; integral to endoplasmic reticulum membrane

Molecular Function:identical protein binding; protein binding; FK506 binding; peptidyl-prolyl cis-trans isomerase activity; metal ion binding

Biological Process: smoothened signaling pathway; camera-type eye development; protein peptidyl-prolyl isomerization; viral reproduction; apoptosis; regulation of gene expression; multicellular organism growth; cell fate specification; positive regulation of BMP signaling pathway; dorsoventral neural tube patterning; negative regulation of apoptosis

NCBI Summary:The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function. [provided by RefSeq, Jul 2008]
UniProt Code:Q14318
NCBI GenInfo Identifier:193806337
NCBI Gene ID:23770
NCBI Accession:Q14318.2
UniProt Related Accession:Q14318
Molecular Weight:55kDa
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase FKBP8
NCBI Synonym Full Names:FKBP prolyl isomerase 8
NCBI Official Symbol:FKBP8  
NCBI Official Synonym Symbols:FKBP38; FKBPr38  
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase FKBP8
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase FKBP8
UniProt Synonym Protein Names:38 kDa FK506-binding protein; 38 kDa FKBP; FKBP-38; hFKBP38; FK506-binding protein 8; FKBP-8; FKBPR38; Rotamase
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:FKBP8  
UniProt Entry Name:FKBP8_HUMAN
Anti-FKBP8 Antibody (CAB7085)Western blot analysis of extracts of various cell lines, using FKBP8 antibody (CAB7085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-FKBP8 Antibody (CAB7085)Immunofluorescence analysis of A549 cells using FKBP8 antibody (CAB7085). Blue: DAPI for nuclear staining.
Anti-FKBP8 Antibody (CAB7085)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FKBP8 antibody (CAB7085). Western blot was performed from the immunoprecipitate using FKBP8 antibody (CAB7085) at a dilition of 1:1000.

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