Anti-FKBP14 항체 (CAB13221)

Antibody Name:	Anti-FKBP14 Antibody
Antibody SKU:	CAB13221
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-211 of human FKBP14 (NP_060416.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, HeLa, U-87MG, MCF7, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-211 of human FKBP14 (NP_060416.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALIP EPEV KIEV LQKP FICH RKTK GGDL MLVH YEGY LEKD GSLF HSTH KHNN GQPI WFTL GILE ALKG WDQG LKGM CVGE KRKL IIPP ALGY GKEG KGKI PPES TLIF NIDL LEIR NGPR SHES FQEM DLND DWKL SKDE VKAY LKKE FEKH GAVV NESH HDAL VEDI FDKE DEDK DGFI SARE FTYK HDEL
Gene ID:	55033
Uniprot:	Q9NWM8
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	24kDa
Observed MW:	22kDa

Synonyms:

FKBP14, EDSKMH, FKBP22, IPBP12

Background:

The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene.

UniProt Protein Function:	FKBP14: PPIases accelerate the folding of proteins during protein synthesis. Defects in FKBP14 are the cause of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss (EDSKMH). A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI- related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand. Clinically, this disorder is characterized by the following features: severe generalized hypotonia at birth with marked muscle weakness that improve in infancy; early-onset progressive kyphoscoliosis; joint hypermobility without contractures; hyperelastic skin with follicular hyperkeratosis, easy bruising, and occasional abnormal scarring; myopathy as confirmed by muscle MRI, histology, and electron microscopy; hearing impairment, which is predominantly sensorineural; and a normal ratio of lysyl pyridinoline to hydroxylysyl pyridinoline (LP/HP) in urine.
UniProt Protein Details:	Protein type:Secreted; EC 5.2.1.8; Isomerase; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7p14.3 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen Molecular Function:FK506 binding; peptidyl-prolyl cis-trans isomerase activity; calcium ion binding Biological Process: protein peptidyl-prolyl isomerization; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response Disease: Ehlers-danlos Syndrome With Progressive Kyphoscoliosis, Myopathy, And Hearing Loss
NCBI Summary:	The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	Q9NWM8
NCBI GenInfo Identifier:	23821568
NCBI Gene ID:	55033
NCBI Accession:	Q9NWM8.1
UniProt Related Accession:	Q9NWM8
Molecular Weight:	24,172 Da
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase FKBP14
NCBI Synonym Full Names:	FK506 binding protein 14, 22 kDa
NCBI Official Symbol:	FKBP14
NCBI Official Synonym Symbols:	EDSKMH; FKBP22; IPBP12
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase FKBP14; FKBP-22; rotamase; 22 kDa FKBP; PPIase FKBP14; FK506-binding protein 14; 22 kDa FK506-binding protein
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase FKBP14
UniProt Synonym Protein Names:	22 kDa FK506-binding protein; 22 kDa FKBP; FKBP-22; FK506-binding protein 14; FKBP-14; Rotamase
UniProt Gene Name:	FKBP14
UniProt Entry Name:	FKB14_HUMAN

Western blot analysis of extracts of various cell lines, using FKBP14 antibody (CAB13221) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

FKBP14 Antibody (PACO09302)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)