Anti-Fibronectin Antibody (CAB16678)

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SKU:
CAB16678
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:Anti-Fibronectin Antibody
Antibody SKU:CAB16678
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin (NP_002017.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin (NP_002017.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLCQ CLGF GSGH FRCD SSRW CHDN GVNY KIGE KWDR QGEN GQMM SCTC LGNG KGEF KCDP HEAT CYDD GKTY HVGE QWQK EYLG AICS CTCF GGQR GWRC DNCR RPGG EPSP EGTT GQSY NQYS QRYH QRTN TNVN CPIE CFMP LDVQ ADRE DSRE
Gene ID:2335
Uniprot:P02751
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:71-73kDa/221-272kDa
Observed MW:290kDa
Synonyms:FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, fibronectin
Background:This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
UniProt Protein Function:FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; extracellular region; ER-Golgi intermediate compartment; basal lamina

Molecular Function:heparin binding; collagen binding; integrin binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; response to wounding; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency

NCBI Summary:This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P02751
NCBI GenInfo Identifier:300669710
NCBI Gene ID:2335
NCBI Accession:P02751.4
UniProt Secondary Accession:P02751,O95609, O95610, Q14312, Q14325, Q14326, Q17RV7 Q564H7, Q585T2, B7ZLF0, E9PE77, E9PG29,
UniProt Related Accession:P02751
Molecular Weight:256,502 Da
NCBI Full Name:Fibronectin
NCBI Synonym Full Names:fibronectin 1
NCBI Official Symbol:FN1  
NCBI Official Synonym Symbols:FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2  
NCBI Protein Information:fibronectin; cold-insoluble globulin; migration-stimulating factor
UniProt Protein Name:Fibronectin
UniProt Synonym Protein Names:Cold-insoluble globulin; CIG
Protein Family:Fibronectin
UniProt Gene Name:FN1  
UniProt Entry Name:FINC_HUMAN
Anti-Fibronectin Antibody (CAB16678)Western blot analysis of extracts of various cell lines, using Fibronectin antibody (CAB16678) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Fibronectin Antibody (CAB16678)Immunohistochemistry of paraffin-embedded human lung cancer using Fibronectin antibody (CAB16678) at dilution of 1:100 (40x lens).
Anti-Fibronectin Antibody (CAB16678)Immunohistochemistry of paraffin-embedded mouse testis leydig using Fibronectin antibody (CAB16678) at dilution of 1:100 (40x lens).
Anti-Fibronectin Antibody (CAB16678)Immunofluorescence analysis of HeLa cells using Fibronectin antibody (CAB16678) at dilution of 1:100. Blue: DAPI for nuclear staining.

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