Anti-FHL1 Antibody (CAB5460)
- SKU:
- CAB5460
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-FHL1 Antibody |
| Antibody SKU: | CAB5460 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, 22Rv1, MCF7, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RDPL QGKK YVQK DGHH CCLK CFDK FCAN TCVE CRKP IGAD SKEV HYKN RFWH DTCF RCAK CLHP LANE TFVA KDNK ILCN KCTT REDS PKCK GCFK AIVA GDQN VEYK GTVW HKDC FTCS NCKQ VIGT GSFF PKGE DFYC VTCH ETKF AKHC VKCN KAIT SGGI TYQD QPWH ADCF VCVT CSKK LAGQ RFTA VEDQ YYCV DCYK NFVA KKCA GCKN PITG FGKG SSVV AYEG QSWH DYCF HCKK CSVN LANK RFVF HQEQ VYCP DCAK KL |
| Gene ID: | 2273 |
| Uniprot: | Q13642 |
| Cellular Location: | Cytoplasm, Nucleus, cytosol |
| Calculated MW: | 22kDa/31kDa/33kDa/34kDa/36kDa |
| Observed MW: | 40kDa |
| Synonyms: | FHL1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA |
| Background: | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described. |
| UniProt Protein Function: | May have an involvement in muscle development or hypertrophy. |
| NCBI Summary: | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009] |
| UniProt Code: | Q13642 |
| NCBI GenInfo Identifier: | 59800384 |
| NCBI Gene ID: | 2273 |
| NCBI Accession: | Q13642.4 |
| UniProt Secondary Accession: | Q13642,O95212, Q13230, Q13645, Q5JXI7, Q5M7Y6, Q6IB30 Q9NZ40, Q9UKZ8, Q9Y630, B7Z5T4, B7Z793, |
| UniProt Related Accession: | Q13642 |
| Molecular Weight: | 33,579 Da |
| NCBI Full Name: | Four and a half LIM domains protein 1 |
| NCBI Synonym Full Names: | four and a half LIM domains 1 |
| NCBI Official Symbol: | FHL1 |
| NCBI Official Synonym Symbols: | KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER |
| NCBI Protein Information: | four and a half LIM domains protein 1 |
| UniProt Protein Name: | Four and a half LIM domains protein 1 |
| UniProt Synonym Protein Names: | Skeletal muscle LIM-protein 1; SLIM; SLIM-1 |
| Protein Family: | Fork head transcription factor |
| UniProt Gene Name: | FHL1 |
| UniProt Entry Name: | FHL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FHL1 antibody (CAB5460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using FHL1 Antibody (CAB5460) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using FHL1 Antibody (CAB5460) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using FHL1 antibody (CAB5460). Blue: DAPI for nuclear staining. |
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