Anti-FHIT Antibody (CAB1196)
- SKU:
- CAB1196
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-FHIT Antibody |
| Antibody SKU: | CAB1196 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HeLa, Jurkat, 293T, Mouse liver, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LVNR KPVV PGHV LVCP LRPV ERFH DLRP DEVA DLFQ TTQR VGTV VEKH FHGT SLTF SMQD GPEA GQTV KHVH VHVL PRKA GDFH RNDS IYEE LQKH DKED FPAS WRSE EEMA AEAA ALRV YFQ |
| Gene ID: | 2272 |
| Uniprot: | P49789 |
| Cellular Location: | Cytoplasm, Mitochondrion, Nucleus |
| Calculated MW: | 16kDa |
| Observed MW: | 17kDa |
| Synonyms: | FHIT, AP3Aase, FRA3B |
| Background: | This gene, a member of the histidine triad gene family, encodes a diadenosine 5', 5'''-P1, P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. |
| UniProt Protein Details: | Protein type:EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; DNA replication; Nucleotide Metabolism - purine; Tumor suppressor Chromosomal Location of Human Ortholog: 3p14.2 Cellular Component: cytoplasm; cytosol Molecular Function:bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; protein binding; ubiquitin protein ligase binding Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process |
| NCBI Summary: | This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P49789 |
| NCBI GenInfo Identifier: | 1706794 |
| NCBI Gene ID: | 2272 |
| NCBI Accession: | P49789.3 |
| UniProt Secondary Accession: | P49789,Q45QG9, Q6IU12, A2IAS9, A2IAT0, A2IAT6, A8K1A9 |
| UniProt Related Accession: | P49789 |
| Molecular Weight: | 16,858 Da |
| NCBI Full Name: | Bis(5'-adenosyl)-triphosphatase |
| NCBI Synonym Full Names: | fragile histidine triad |
| NCBI Official Symbol: | FHIT |
| NCBI Official Synonym Symbols: | FRA3B; AP3Aase |
| NCBI Protein Information: | bis(5'-adenosyl)-triphosphatase |
| UniProt Protein Name: | Bis(5'-adenosyl)-triphosphatase |
| UniProt Synonym Protein Names: | AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein |
| Protein Family: | Bis(5'-adenosyl)-triphosphatase |
| UniProt Gene Name: | FHIT |
| UniProt Entry Name: | FHIT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FHIT antibody (CAB1196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat heart using FHIT antibody (CAB1196) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using FHIT antibody (CAB1196) at dilution of 1:100 (40x lens). |
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