Anti-FGF9 Antibody (CAB6374)
- SKU:
- CAB6374
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-FGF9 Antibody |
| Antibody SKU: | CAB6374 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FGF9 (NP_002001.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, 293T, HepG2, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FGF9 (NP_002001.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPL GEVG NYFG VQDA VPFG NVPV LPVD SPVL LSDH LGQS EAGG LPRG PAVT DLDH LKGI LRRR QLYC RTGF HLEI FPNG TIQG TRKD HSRF GILE FISI AVGL VSIR GVDS GLYL GMNE KGEL YGSE KLTQ ECVF REQF EENW YNTY SSNL YKHV DTGR RYYV ALNK DGTP REGT RTKR HQKF THFL PRPV DPDK VPEL YKDI LSQS |
| Gene ID: | 2254 |
| Uniprot: | P31371 |
| Cellular Location: | Secreted |
| Calculated MW: | 23kDa |
| Observed MW: | 55kDa/23kDa |
| Synonyms: | FGF9, FGF-9, GAF, HBFG-9, HBGF-9, SYNS3 |
| Background: | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. |
| UniProt Protein Function: | FGF9: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Belongs to the heparin-binding growth factors family. |
| UniProt Protein Details: | Protein type:Cytokine Chromosomal Location of Human Ortholog: 13q11-q12 Cellular Component: extracellular space; cytoplasm; extracellular region; basement membrane Molecular Function:heparin binding; growth factor activity; fibroblast growth factor receptor binding Biological Process: negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic skeletal development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of activin receptor signaling pathway; substantia nigra development; cell-cell signaling; positive regulation of MAPKKK cascade; protein import into nucleus; male sex determination; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of smoothened signaling pathway; chondrocyte differentiation; angiogenesis; regulation of timing of cell differentiation; embryonic gut development; positive regulation of cardiac muscle cell proliferation; embryonic limb morphogenesis; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; male gonad development; osteoblast differentiation; positive regulation of cell division; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation Disease: Multiple Synostoses Syndrome 3 |
| NCBI Summary: | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P31371 |
| NCBI GenInfo Identifier: | 544290 |
| NCBI Gene ID: | 2254 |
| NCBI Accession: | P31371.3 |
| UniProt Secondary Accession: | P31371,Q3SY32, A8K427, |
| UniProt Related Accession: | P31371 |
| Molecular Weight: | 23,441 Da |
| NCBI Full Name: | Fibroblast growth factor 9 |
| NCBI Synonym Full Names: | fibroblast growth factor 9 |
| NCBI Official Symbol: | FGF9 |
| NCBI Official Synonym Symbols: | GAF; FGF-9; SYNS3; HBFG-9; HBGF-9 |
| NCBI Protein Information: | fibroblast growth factor 9; heparin-binding growth factor 9; fibroblast growth factor 9 (glia-activating factor) |
| UniProt Protein Name: | Fibroblast growth factor 9 |
| UniProt Synonym Protein Names: | Glia-activating factor; GAF; Heparin-binding growth factor 9; HBGF-9 |
| Protein Family: | Fibroblast growth factor |
| UniProt Gene Name: | FGF9 |
| UniProt Entry Name: | FGF9_HUMAN |
 | Western blot analysis of extracts of Mouse kidney, using FGF9 antibody (CAB6374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using FGF9 antibody (CAB6374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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