Anti-FER Antibody (CAB9687)
- SKU:
- CAB9687
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FER Antibody |
| Antibody SKU: | CAB9687 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-450 of human FER (NP_005237.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HT-1080, SW480, LO2, NIH/3T3, Mouse liver, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-450 of human FER (NP_005237.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YDIT LPLL LDSL QKMQ EEMI KALK GIFD EYSQ ITSL VTEE IVNV HKEI QMSV EQID PSTE YNNF IDVH RTTA AKEQ EIEF DTSL LEEN ENLQ ANEI MWNN LTAE SLQV MLKT LAEE LMQT QQML LNKE EAVL ELEK RIEE SSET CEKK SDIV LLLS QKQA LEEL KQSV QQLR CTEA KFSA QKEL LEQK VQEN DGKE PPPV VNYE EDAR SVTS MERK ERLS KFES IRHS IAGI IRSP KSAL GSSA LSDM ISI |
| Gene ID: | 2241 |
| Uniprot: | P16591 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Nucleus, Peripheral membrane protein, cell cortex, cytoskeleton |
| Calculated MW: | 51kDa/74kDa/94kDa |
| Observed MW: | 110kDa |
| Synonyms: | FER, PPP1R74, TYK3, p94-Fer |
| Background: | The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. |
| UniProt Protein Function: | Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth. |
| UniProt Protein Details: | Protein type:Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Fer family Chromosomal Location of Human Ortholog: 5q21 Cellular Component: actin cytoskeleton; cell cortex; cell junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; lamellipodium; microtubule cytoskeleton; nuclear chromatin; nucleus Molecular Function:ATP binding; epidermal growth factor receptor binding; lipid binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase 1 binding; protein-tyrosine kinase activity Biological Process: actin cytoskeleton reorganization; activation of NF-kappaB transcription factor; cell adhesion; cell differentiation; cell proliferation; cellular response to insulin stimulus; chemotaxis; cytokine and chemokine mediated signaling pathway; diapedesis; innate immune response; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; negative regulation of mast cell activation during immune response; peptidyl-tyrosine phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of actin filament polymerization; positive regulation of cell migration; positive regulation of cell proliferation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of epidermal growth factor receptor signaling pathway; regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; response to lipopolysaccharide; tyrosine phosphorylation of Stat3 protein |
| NCBI Summary: | The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015] |
| UniProt Code: | P16591 |
| NCBI GenInfo Identifier: | 97536202 |
| NCBI Gene ID: | 2241 |
| NCBI Accession: | P16591.2 |
| UniProt Secondary Accession: | P16591,B2RCR4, B4DSQ2, H2FLB8, |
| UniProt Related Accession: | P16591 |
| Molecular Weight: | 51,643 Da |
| NCBI Full Name: | Tyrosine-protein kinase Fer |
| NCBI Synonym Full Names: | FER tyrosine kinase |
| NCBI Official Symbol: | FER |
| NCBI Official Synonym Symbols: | TYK3; PPP1R74; p94-Fer |
| NCBI Protein Information: | tyrosine-protein kinase Fer |
| UniProt Protein Name: | Tyrosine-protein kinase Fer |
| UniProt Synonym Protein Names: | Feline encephalitis virus-related kinase FER; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; Proto-oncogene c-Fer; Tyrosine kinase 3; p94-Fer |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | FER |
| UniProt Entry Name: | FER_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FER antibody (CAB9687) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 10s. |
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