Anti-FDPS Antibody (CAB5744)
- SKU:
- CAB5744
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-FDPS Antibody |
| Antibody SKU: | CAB5744 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-419 of human FDPS (NP_001129293.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, HeLa, MCF7, U-251MG, BxPC-3, Mouse liver, Mouse ovary, Rat liver, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-419 of human FDPS (NP_001129293.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IGGK YNRG LTVV VAFR ELVE PRKQ DADS LQRA WTVG WCVE LLQA FFLV ADDI MDSS LTRR GQIC WYQK PGVG LDAI NDAN LLEA CIYR LLKL YCRE QPYY LNLI ELFL QSSY QTEI GQTL DLLT APQG NVDL VRFT EKRY KSIV KYKT AFYS FYLP IAAA MYMA GIDG EKEH ANAK KILL EMGE FFQI QDDY LDLF GDPS VTGK IGTD IQDN KCSW LVVQ CLQR ATPE QYQI LKEN YGQK EAEK VARV KALY EELD LPAV FLQY EEDS YSHI MALI EQYA APLP PAVF LGLA RKIY KRRK |
| Gene ID: | 2224 |
| Uniprot: | P14324 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 40kDa/48kDa |
| Observed MW: | 38kDa |
| Synonyms: | FDPS, FPPS, FPS, POROK9 |
| Background: | This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FDPS: Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. Belongs to the FPP/GGPP synthase family. |
| UniProt Protein Details: | Protein type:EC 2.5.1.10; Transferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.5.1.1; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q22 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol Molecular Function:geranyltranstransferase activity; dimethylallyltranstransferase activity; metal ion binding Biological Process: viral reproduction; geranyl diphosphate biosynthetic process; farnesyl diphosphate biosynthetic process; cholesterol biosynthetic process |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008] |
| UniProt Code: | P14324 |
| NCBI GenInfo Identifier: | 215274250 |
| NCBI Gene ID: | 2224 |
| NCBI Accession: | P14324.4 |
| UniProt Secondary Accession: | P14324,Q96G29, D3DV91, E9PCI9, |
| UniProt Related Accession: | P14324 |
| Molecular Weight: | 40,532 Da |
| NCBI Full Name: | Farnesyl pyrophosphate synthase |
| NCBI Synonym Full Names: | farnesyl diphosphate synthase |
| NCBI Official Symbol: | FDPS |
| NCBI Official Synonym Symbols: | FPS; FPPS |
| NCBI Protein Information: | farnesyl pyrophosphate synthase; FPP synthase; FPP synthetase; geranyltranstransferase; dimethylallyltranstransferase; (2E,6E)-farnesyl diphosphate synthase; farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase |
| UniProt Protein Name: | Farnesyl pyrophosphate synthase |
| UniProt Synonym Protein Names: | (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase (EC:2.5.1.1); Farnesyl diphosphate synthase; Geranyltranstransferase |
| Protein Family: | Farnesyl diphosphate synthase |
| UniProt Gene Name: | FDPS |
| UniProt Entry Name: | FPPS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FDPS antibody (CAB5744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using FDPS antibody (CAB5744). Blue: DAPI for nuclear staining. |
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