Anti-FBXO21 Antibody (CAB16107)
- SKU:
- CAB16107
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-FBXO21 Antibody |
| Antibody SKU: | CAB16107 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 440-621 of human FBXO21 (NP_055817.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, MCF7, Mouse liver, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 440-621 of human FBXO21 (NP_055817.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PEKV LDIL QHIQ TLDP GQHG AVGY LVQH TLEH IERK KEEV GVEV KLRS DEKH RDVC YSIG LIMK HKRY GYNC VIYG WDPT CMMG HEWI RNMN VHSL PHGH HQPF YNVL VEDG SCRY AAQE NLEY NVEP QEIS HPDV GRYF SEFT GTHY IPNA ELEI RYPE DLEF VYET VQNI YSAK KENI DE |
| Gene ID: | 23014 |
| Uniprot: | O94952 |
| Cellular Location: | |
| Calculated MW: | 71kDa/72kDa |
| Observed MW: | 72kDa |
| Synonyms: | FBXO21, FBX21 |
| Background: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. |
| UniProt Protein Function: | FBXO21: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 12q24.22 |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94952 |
| NCBI GenInfo Identifier: | 218512038 |
| NCBI Gene ID: | 23014 |
| NCBI Accession: | O94952.2 |
| UniProt Secondary Accession: | O94952,Q5BJG0, Q9H087, B3KMF0, |
| UniProt Related Accession: | O94952 |
| Molecular Weight: | 71,479 Da |
| NCBI Full Name: | F-box only protein 21 |
| NCBI Synonym Full Names: | F-box protein 21 |
| NCBI Official Symbol: | FBXO21 |
| NCBI Official Synonym Symbols: | FBX21 |
| NCBI Protein Information: | F-box only protein 21 |
| UniProt Protein Name: | F-box only protein 21 |
| Protein Family: | F-box only protein |
| UniProt Gene Name: | FBXO21 |
| UniProt Entry Name: | FBX21_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXO21 antibody (CAB16107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat lung using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using FBXO21 antibody (CAB16107) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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