Anti-FBLIM1 Antibody (CAB15850)
- SKU:
- CAB15850
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-FBLIM1 Antibody |
| Antibody SKU: | CAB15850 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-272 of human FBLIM1 (NP_060026.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-272 of human FBLIM1 (NP_060026.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TDIC AFCH KTVS PREL AVEA MKRQ YHAQ CFTC RTCR RQLA GQSF YQKD GRPL CEPC YQDT LERC GKCG EVVR DHII RALG QAFH PSCF TCVT C |
| Gene ID: | 54751 |
| Uniprot: | Q8WUP2 |
| Cellular Location: | Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion |
| Calculated MW: | 30kDa/40kDa |
| Observed MW: | 41kDa |
| Synonyms: | FBLIM1, CAL, FBLP-1, FBLP1 |
| Background: | This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p36.21 Cellular Component: cytosol; focal adhesion; stress fiber Molecular Function:filamin binding; protein binding Biological Process: cell-cell adhesion; regulation of integrin activation |
| NCBI Summary: | This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WUP2 |
| NCBI GenInfo Identifier: | 125987829 |
| NCBI Gene ID: | 54751 |
| NCBI Accession: | Q8WUP2.2 |
| UniProt Secondary Accession: | Q8WUP2,Q5VVE0, Q5VVE1, Q8IX23, Q96T00, Q9UFD6, B3KNY0 |
| UniProt Related Accession: | Q8WUP2 |
| Molecular Weight: | 30,770 Da |
| NCBI Full Name: | Filamin-binding LIM protein 1 |
| NCBI Synonym Full Names: | filamin binding LIM protein 1 |
| NCBI Official Symbol: | FBLIM1 |
| NCBI Official Synonym Symbols: | CAL; FBLP1; FBLP-1 |
| NCBI Protein Information: | filamin-binding LIM protein 1 |
| UniProt Protein Name: | Filamin-binding LIM protein 1 |
| UniProt Synonym Protein Names: | Migfilin; Mitogen-inducible 2-interacting protein; MIG2-interacting protein |
| Protein Family: | Filamin-binding LIM protein |
| UniProt Gene Name: | FBLIM1 |
| UniProt Entry Name: | FBLI1_HUMAN |
 | Western blot analysis of extracts of rat heart, using FBLIM1 antibody (CAB15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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