Anti-FARSA 항체 (CAB15670)

Antibody Name:	Anti-FARSA Antibody
Antibody SKU:	CAB15670
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-486 of human FARSA (NP_004452.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-486 of human FARSA (NP_004452.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YNFL AHGV LPDS GHLH PLLK VRSQ FRQI FLEM GFTE MPTD NFIE SSFW NFDA LFQP QQHP ARDQ HDTF FLRD PAEA LQLP MDYV QRVK RTHS QGGY GSQG YKYN WKLD EARK NLLR THTT SASA RALY RLAQ KKPF TPVK YFSI DRVF RNET LDAT HLAE FHQI EGVV ADHG LTLG HLMG VLRE FFTK LGIT QLRF KPAY NPYT EPSM EVFS YHQG LKKW VEVG NSGV FRPE MLLP MGLP ENVS VIAW GLSL ERPT MIKY GINN IREL VGHK VNLQ M
Gene ID:	2193
Uniprot:	Q9Y285
Cellular Location:	Cytoplasm
Calculated MW:	54kDa/57kDa
Observed MW:	58kDa

Synonyms:

FARSA, CML33, FARSL, FARSLA, FRSA, PheHA

Background:

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression

UniProt Protein Function:	FARSLA: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; EC 6.1.1.20; Ligase; RNA-binding Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; phenylalanine-tRNA ligase activity; ATP binding; tRNA binding Biological Process: tRNA aminoacylation for protein translation; phenylalanyl-tRNA aminoacylation; gene expression
NCBI Summary:	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y285
NCBI GenInfo Identifier:	12643946
NCBI Gene ID:	2193
NCBI Accession:	Q9Y285.3
UniProt Secondary Accession:	Q9Y285,Q9NSD8, Q9Y4W8, B4E363,
UniProt Related Accession:	Q9Y285
Molecular Weight:	508
NCBI Full Name:	Phenylalanine--tRNA ligase alpha subunit
NCBI Synonym Full Names:	phenylalanyl-tRNA synthetase, alpha subunit
NCBI Official Symbol:	FARSA
NCBI Official Synonym Symbols:	FRSA; CML33; FARSL; PheHA; FARSLA
NCBI Protein Information:	phenylalanine--tRNA ligase alpha subunit; pheRS; phenylalanine--tRNA ligase alpha chain; phenylalanyl-tRNA synthetase alpha chain; phenylalanine-tRNA synthetase alpha-subunit; phenylalanine tRNA ligase 1, alpha, cytoplasmic; phenylalanyl-tRNA synthetase-l
UniProt Protein Name:	Phenylalanine--tRNA ligase alpha subunit
UniProt Synonym Protein Names:	CML33; Phenylalanyl-tRNA synthetase alpha subunit; PheRS
Protein Family:	Phenylalanine--tRNA ligase
UniProt Gene Name:	FARSA
UniProt Entry Name:	SYFA_HUMAN

Western blot analysis of extracts of various cell lines, using FARSA antibody (CAB15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

FARSA Antibody (PACO09235)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)