Anti-FAR1 항체 (CAB16284)

Antibody Name:	Anti-FAR1 Antibody
Antibody SKU:	CAB16284
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FAR1 (NP_115604.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FAR1 (NP_115604.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVSI PEYY EGKN VLLT GATG FLGK VLLE KLLR SCPK VNSV YVLV RQKA GQTP QERV EEVL SGKL FDRL RDEN PDFR EKII AINS ELTQ PKLA LSEE DKEV IIDS TNII FHCA ATVR FNEN LRDA VQLN VIAT RQLI LLAQ QMKN LEVF MHVS TAYA YCNR KHID EVVY PPPV DPKK LIDS LEWM DDGL VNDI TPKL IGDR PNTY IYTK ALAE YVVQ QEGA
Gene ID:	84188
Uniprot:	Q8WVX9
Cellular Location:
Calculated MW:	59kDa
Observed MW:	Refer to figures

Synonyms:

FAR1, MLSTD2, PFCRD, SDR10E1

Background:

The protein encoded by this gene is required for the reduction of fatty acids to fatty alcohols, a process that is required for the synthesis of monoesters and ether lipids. NADPH is required as a cofactor in this reaction, and 16-18 carbon saturated and unsaturated fatty acids are the preferred substrate. This is a peroxisomal membrane protein, and studies suggest that the N-terminus contains a large catalytic domain located on the outside of the peroxisome, while the C-terminus is exposed to the matrix of the peroxisome. Studies indicate that the regulation of this protein is dependent on plasmalogen levels. Mutations in this gene have been associated with individuals affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity (PMID: 25439727). A pseudogene of this gene is located on chromosome 13.

UniProt Protein Function:	MLSTD2: Catalyzes the reduction of saturated fatty acyl-CoA with chain length C16 or C18 to fatty alcohols. Belongs to the fatty acyl-CoA reductase family.
UniProt Protein Details:	Protein type:Oxidoreductase; Membrane protein, integral; EC 1.2.1.n2 Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: peroxisomal matrix; peroxisomal membrane; peroxisome Molecular Function:oxidoreductase activity Biological Process: ether lipid biosynthetic process; glycerophospholipid biosynthetic process; wax biosynthetic process Disease: Peroxisomal Fatty Acyl-coa Reductase 1 Disorder
NCBI Summary:	The protein encoded by this gene is required for the reduction of fatty acids to fatty alcohols, a process that is required for the synthesis of monoesters and ether lipids. NADPH is required as a cofactor in this reaction, and 16-18 carbon saturated and unsaturated fatty acids are the preferred substrate. This is a peroxisomal membrane protein, and studies suggest that the N-terminus contains a large catalytic domain located on the outside of the peroxisome, while the C-terminus is exposed to the matrix of the peroxisome. Studies indicate that the regulation of this protein is dependent on plasmalogen levels. Mutations in this gene have been associated with individuals affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity (PMID: 25439727). A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jan 2015]
UniProt Code:	Q8WVX9
NCBI GenInfo Identifier:	33872399
NCBI Gene ID:	84188
NCBI Accession:	BC017377
UniProt Secondary Accession:	Q8WVX9,Q5CZA3, D3DQW8,
UniProt Related Accession:	Q8WVX9
Molecular Weight:	59,357 Da
NCBI Full Name:	Homo sapiens fatty acyl CoA reductase 1, mRNA
NCBI Synonym Full Names:	fatty acyl-CoA reductase 1
NCBI Official Symbol:	FAR1
NCBI Official Synonym Symbols:	PFCRD; MLSTD2; SDR10E1
NCBI Protein Information:	fatty acyl-CoA reductase 1
UniProt Protein Name:	Fatty acyl-CoA reductase 1
UniProt Synonym Protein Names:	Male sterility domain-containing protein 2
Protein Family:	Fatty acyl-CoA reductase
UniProt Gene Name:	FAR1
UniProt Entry Name:	FACR1_HUMAN

Western blot analysis of extracts of various cell lines, using FAR1 antibody (CAB16284) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)