Anti-FADS2 Antibody (CAB10270)
- SKU:
- CAB10270
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-FADS2 Antibody |
| Antibody SKU: | CAB10270 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human FADS2 (NP_004256.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, A-549, U-87MG, HeLa, Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human FADS2 (NP_004256.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGKG GNQG EGAA EREV SVPT FSWE EIQK HNLR TDRW LVID RKVY NITK WSIQ HPGG QRVI GHYA GEDA TDAF RAFH PDLE FVGK FLKP LLIG ELAP EEPS QDHG KNSK ITED FRAL RKTA EDMN LFKT NHV |
| Gene ID: | 9415 |
| Uniprot: | O95864 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 45kDa/48kDa/49kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | FADS2, D6D, DES6, FADSD6, LLCDL2, SLL0262, TU13 |
| Background: | The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | FADS2: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. Belongs to the fatty acid desaturase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.19.-; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase Chromosomal Location of Human Ortholog: 11q12.2 Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; membrane Molecular Function:linoleoyl-CoA desaturase activity Biological Process: linoleic acid metabolic process |
| NCBI Summary: | The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O95864 |
| NCBI GenInfo Identifier: | 74762131 |
| NCBI Gene ID: | 9415 |
| NCBI Accession: | O95864.1 |
| UniProt Secondary Accession: | O95864,Q6MZQ7, Q96H07, Q96SV8, Q9H3G3, Q9Y3X4, A8K2M6 B7Z634, |
| UniProt Related Accession: | O95864 |
| Molecular Weight: | 52kDa |
| NCBI Full Name: | Fatty acid desaturase 2 |
| NCBI Synonym Full Names: | fatty acid desaturase 2 |
| NCBI Official Symbol: | FADS2 |
| NCBI Official Synonym Symbols: | D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262 |
| NCBI Protein Information: | fatty acid desaturase 2 |
| UniProt Protein Name: | Fatty acid desaturase 2 |
| UniProt Synonym Protein Names: | Acyl-CoA 6-desaturase; Delta(6) fatty acid desaturase; D6D; Delta(6) desaturase; Delta-6 desaturase |
| Protein Family: | Fatty acid desaturase |
| UniProt Gene Name: | FADS2 |
 | Western blot analysis of extracts of various cell lines, using FADS2 antibody (CAB10270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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