Anti-FADD Antibody (CAB5819)
- SKU:
- CAB5819
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-FADD Antibody |
| Antibody SKU: | CAB5819 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, MCF7, HeLa, Mouse liver, Mouse kidney, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDPF LVLL HSVS SSLS SSEL TELK FLCL GRVG KRKL ERVQ SGLD LFSM LLEQ NDLE PGHT ELLR ELLA SLRR HDLL RRVD DFEA GAAA GAAP GEED LCAA FNVI CDNV GKDW RRLA RQLK VSDT KIDS IEDR YPRN LTER VRES LRIW KNTE KENA TVAH LVGA LRSC QMNL VADL VQEV QQAR DLQN RSGA MSPM SWNS DAST SEAS |
| Gene ID: | 8772 |
| Uniprot: | Q13158 |
| Cellular Location: | |
| Calculated MW: | 23kDa |
| Observed MW: | 26kDa |
| Synonyms: | GIG3, MORT1, FADD, FADD |
| Background: | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. |
| UniProt Protein Function: | FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. |
| UniProt Protein Details: | Protein type:Apoptosis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft Molecular Function:identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding Biological Process: viral reproduction; positive regulation of apoptosis; apoptosis; positive regulation of T cell mediated cytotoxicity; positive regulation of proteolysis; protein heterooligomerization; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of adaptive immune response; spleen development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations |
| NCBI Summary: | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13158 |
| NCBI GenInfo Identifier: | 2498355 |
| NCBI Gene ID: | 8772 |
| NCBI Accession: | Q13158.1 |
| UniProt Secondary Accession: | Q13158,Q14866, Q6IBR4, |
| UniProt Related Accession: | Q13158 |
| Molecular Weight: | 208 |
| NCBI Full Name: | FAS-associated death domain protein |
| NCBI Synonym Full Names: | Fas (TNFRSF6)-associated via death domain |
| NCBI Official Symbol: | FADD |
| NCBI Official Synonym Symbols: | GIG3; MORT1 |
| NCBI Protein Information: | FAS-associated death domain protein; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein |
| UniProt Protein Name: | FAS-associated death domain protein |
| UniProt Synonym Protein Names: | FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD |
| Protein Family: | Fas-associated death domain protein |
| UniProt Gene Name: | FADD |
| UniProt Entry Name: | FADD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FADD antibody (CAB5819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunohistochemistry of paraffin-embedded rat kidney using FADD antibody (CAB5819) at dilution of 1:200 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug FADD antibody (CAB5819). Western blot was performed from the immunoprecipitate using FADD antibody (CAB5819) at a dilition of 1:1000. |
Related Products
Anti-FADD Antibody (CAB16459)
Anti-FADD Antibody (CAB19838)
Anti-FADD Antibody (CAB20252)
![Anti-FADD Antibody (CAB18044)[KO Validated]](https://cdn11.bigcommerce.com/s-39x6lpnvxv/images/stencil/590x590/products/22687/20878/anti-fadd-antibody-cab18044ko-validated__99866__23962.1644253210.jpg?c=1 "Anti-FADD Antibody (CAB18044)[KO Validated]")