Anti-FA2H Antibody (CAB13874)
- SKU:
- CAB13874
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-FA2H Antibody |
| Antibody SKU: | CAB13874 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 95-170 of human FA2H (NP_077282.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse stomach, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 95-170 of human FA2H (NP_077282.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NEPV ALEE TQKT DPAM EPRF KVVD WDKD LVDW RKPL LWQV GHLG EKYD EWVH QPVT RPIR LFHS DLIE GLSK TVWY |
| Gene ID: | 79152 |
| Uniprot: | Q7L5A8 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein |
| Calculated MW: | 18kDa/42kDa |
| Observed MW: | 40kDa |
| Synonyms: | FA2H, FAAH, FAH1, FAXDC1, SCS7, SPG35 |
| Background: | This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia. |
| UniProt Protein Function: | FA2H: Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Belongs to the sterol desaturase family. SCS7 subfamily. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 16q23 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Biological Process: fatty acid metabolic process; sphingolipid biosynthetic process Disease: Spastic Paraplegia 35, Autosomal Recessive |
| NCBI Summary: | This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010] |
| UniProt Code: | Q7L5A8 |
| NCBI GenInfo Identifier: | 74749893 |
| NCBI Gene ID: | 79152 |
| NCBI Accession: | Q7L5A8.1 |
| UniProt Secondary Accession: | Q7L5A8,O75213, Q96DK1, Q9H1A5, B7Z8T6, |
| UniProt Related Accession: | Q7L5A8 |
| Molecular Weight: | 18,324 Da |
| NCBI Full Name: | Fatty acid 2-hydroxylase |
| NCBI Synonym Full Names: | fatty acid 2-hydroxylase |
| NCBI Official Symbol: | FA2H |
| NCBI Official Synonym Symbols: | FAAH; FAH1; SCS7; SPG35; FAXDC1 |
| NCBI Protein Information: | fatty acid 2-hydroxylase |
| UniProt Protein Name: | Fatty acid 2-hydroxylase |
| UniProt Synonym Protein Names: | Fatty acid alpha-hydroxylase |
| Protein Family: | Fatty acid 2-hydroxylase |
| UniProt Gene Name: | FA2H |
| UniProt Entry Name: | FA2H_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FA2H antibody (CAB13874) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
Related Products
Anti-FA2H Antibody (CAB13873)
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
