Anti-ESAM Antibody (CAB12210)
- SKU:
- CAB12210
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ESAM Antibody |
| Antibody SKU: | CAB12210 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-248 of human ESAM (NP_620411.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HT-29, HepG2, 293T, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-248 of human ESAM (NP_620411.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QLQL HLPA NRLQ AVEG GEVV LPAW YTLH GEVS SSQP WEVP FVMW FFKQ KEKE DQVL SYIN GVTT SKPG VSLV YSMP SRNL SLRL EGLQ EKDS GPYS CSVN VQDK QGKS RGHS IKTL ELNV LVPP APPS CRLQ GVPH VGAN VTLS CQSP RSKP AVQY QWDR QLPS FQTF FAPA LDVI RGSL SLTN LSSS MAGV YVCK AHNE VGTA QCNV TLEV STGP GAA |
| Gene ID: | 90952 |
| Uniprot: | Q96AP7 |
| Cellular Location: | Cell junction, Cell membrane, Single-pass type I membrane protein, adherens junction, tight junction |
| Calculated MW: | 15kDa/41kDa |
| Observed MW: | 60kDa |
| Synonyms: | ESAM, W117m |
| Background: |
| UniProt Protein Function: | ESAM: Can mediate aggregation most likely through a homophilic molecular interaction. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: plasma membrane Biological Process: leukocyte migration |
| UniProt Code: | Q96AP7 |
| NCBI GenInfo Identifier: | 68052240 |
| NCBI Gene ID: | 90952 |
| NCBI Accession: | Q96AP7.1 |
| UniProt Secondary Accession: | Q96AP7,Q96T50, B4DVN8, |
| UniProt Related Accession: | Q96AP7 |
| Molecular Weight: | 41kDa |
| NCBI Full Name: | Endothelial cell-selective adhesion molecule |
| NCBI Synonym Full Names: | endothelial cell adhesion molecule |
| NCBI Official Symbol: | ESAM |
| NCBI Official Synonym Symbols: | W117m |
| NCBI Protein Information: | endothelial cell-selective adhesion molecule |
| UniProt Protein Name: | Endothelial cell-selective adhesion molecule |
| Protein Family: | Endothelial cell-selective adhesion molecule |
| UniProt Gene Name: | ESAM |
 | Western blot analysis of extracts of various cell lines, using ESAM antibody (CAB12210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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