Anti-ERLIN1 Antibody (CAB14843)
- SKU:
- CAB14843
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ERLIN1 Antibody |
| Antibody SKU: | CAB14843 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, BxPC-3, DU145, OVCAR3, Mouse kidney, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KADA EYYA AHKY ATSN KHKL TPEY LELK KYQA IASN SKIY FGSN IPNM FVDS SCAL KYSD IRTG RESS LPSK EALE PSGE NVIQ NKES TG |
| Gene ID: | 10613 |
| Uniprot: | O75477 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type II membrane protein |
| Calculated MW: | 38kDa |
| Observed MW: | 39kDa |
| Synonyms: | ERLIN1, C10orf69, Erlin-1, KE04, KEO4, SPFH1, SPG62, erlin-1 |
| Background: | The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1, 4, 5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62. |
| UniProt Protein Function: | SPFH1: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Belongs to the band 7/mec-2 family. Forms a heteromeric complex with ERLIN2. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 10q24.31 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; protein complex Molecular Function:cholesterol binding; protein binding Biological Process: ER-associated protein catabolic process; negative regulation of cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process; SREBP-mediated signaling pathway |
| UniProt Code: | O75477 |
| NCBI GenInfo Identifier: | 67461552 |
| NCBI Gene ID: | 10613 |
| NCBI Accession: | O75477.1 |
| UniProt Secondary Accession: | O75477,Q53HV0, B0QZ42, |
| UniProt Related Accession: | O75477 |
| Molecular Weight: | 38,926 Da |
| NCBI Full Name: | Erlin-1 |
| NCBI Synonym Full Names: | ER lipid raft associated 1 |
| NCBI Official Symbol: | ERLIN1 |
| NCBI Official Synonym Symbols: | KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69 |
| NCBI Protein Information: | erlin-1 |
| UniProt Protein Name: | Erlin-1 |
| UniProt Synonym Protein Names: | Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1 |
| Protein Family: | Erlin |
| UniProt Gene Name: | ERLIN1 |
| UniProt Entry Name: | ERLN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (CAB14843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody (CAB14843) at dilution of 1:100 (40x lens). |
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