Anti-Erbin Antibody (CAB8585)
- SKU:
- CAB8585
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Erbin Antibody |
| Antibody SKU: | CAB8585 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1152-1371 of human ERBB2IP (NP_061165.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1152-1371 of human ERBB2IP (NP_061165.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IPER TMSV SDFN YSRT SPSK RPNA RVGS EHSL LDPP GKSK VPRD WREQ VLRH IEAK KLEK MPLS NGQM GQPL RPQA NYSQ IHHP PQAS VARH PSRE QLID YLML KVAH QPPY TQPH CSPR QGHE LAKQ EIRV RVEK DPEL GFSI SGGV GGRG NPFR PDDD GIFV TRVQ PEGP ASKL LQPG DKII QANG YSFI NIEH GQAV SLLK TFQN TVEL IIVR EVSS |
| Gene ID: | 55914 |
| Uniprot: | Q96RT1 |
| Cellular Location: | Basolateral cell membrane, Cell junction, Nucleus membrane, hemidesmosome |
| Calculated MW: | 146-159kDa |
| Observed MW: | 182kDa |
| Synonyms: | ERBIN, ERBB2IP, HEL-S-78, LAP2, erbin |
| Background: | This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | Erbin: Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state. Belongs to the LAP (LRR and PDZ) protein family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 5q12.3 Cellular Component: nucleoplasm; nuclear membrane; hemidesmosome; cytoplasm; basal plasma membrane; plasma membrane; basement membrane; cell junction; nucleus Molecular Function:integrin binding; ErbB-2 class receptor binding; protein binding; structural constituent of cytoskeleton Biological Process: integrin-mediated signaling pathway; establishment and/or maintenance of epithelial cell polarity; epidermal growth factor receptor signaling pathway; basal protein localization; intermediate filament cytoskeleton organization and biogenesis; cell adhesion; cell growth; signal transduction; cell cycle; protein targeting |
| NCBI Summary: | This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q96RT1 |
| NCBI GenInfo Identifier: | 8923909 |
| NCBI Gene ID: | 55914 |
| NCBI Accession: | NP_061165.1 |
| UniProt Related Accession: | Q96RT1 |
| Molecular Weight: | |
| NCBI Full Name: | erbin isoform 2 |
| NCBI Synonym Full Names: | erbb2 interacting protein |
| NCBI Official Symbol: | ERBIN |
| NCBI Official Synonym Symbols: | LAP2; ERBB2IP; HEL-S-78 |
| NCBI Protein Information: | erbin |
| UniProt Protein Name: | Protein LAP2 |
| UniProt Synonym Protein Names: | Densin-180-like protein; Erbb2-interacting protein; Erbin |
| Protein Family: | Leucine aminopeptidase |
| UniProt Gene Name: | ERBB2IP |
| UniProt Entry Name: | LAP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERBB2IP antibody (CAB8585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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