Anti-EPRS Antibody (CAB15245)
- SKU:
- CAB15245
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EPRS Antibody |
| Antibody SKU: | CAB15245 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | 293T, HeLa |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EAKV LFDK VASQ GEVV RKLK TEKA PKDQ VDIA VQEL LQLK AQYK SLIG VEYK PVSA TGAE DKDK KKKE KENK SEKQ NKPQ KQND GQRK DPSK NQGG GLSS S |
| Gene ID: | 2058 |
| Uniprot: | P07814 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 170kDa |
| Observed MW: | 190kDa |
| Synonyms: | EPRS, EARS, GLUPRORS, PARS, PIG32, QARS, QPRS |
| Background: | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. |
| UniProt Protein Function: | EPRS: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L. |
| UniProt Protein Details: | Protein type:Translation; EC 6.1.1.17; Ligase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 6.1.1.15 Chromosomal Location of Human Ortholog: 1q41 Cellular Component: cytoplasm; cytosol; membrane; ribonucleoprotein complex Molecular Function:glutamate-tRNA ligase activity; GTPase binding; proline-tRNA ligase activity; protein binding Biological Process: negative regulation of translation; prolyl-tRNA aminoacylation; protein complex assembly; tRNA aminoacylation for protein translation |
| NCBI Summary: | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07814 |
| NCBI GenInfo Identifier: | 288558855 |
| NCBI Gene ID: | 2058 |
| NCBI Accession: | P07814.5 |
| UniProt Secondary Accession: | P07814,Q05BP6, Q05DF8, Q5DSM1, Q5H9S5, Q6PD57, Q86X73 A0AVA9, B9EGH3, |
| UniProt Related Accession: | P07814 |
| Molecular Weight: | 170,591 Da |
| NCBI Full Name: | Bifunctional glutamate/proline--tRNA ligase |
| NCBI Synonym Full Names: | glutamyl-prolyl-tRNA synthetase |
| NCBI Official Symbol: | EPRS |
| NCBI Official Synonym Symbols: | EARS; PARS; QARS; QPRS; PIG32; GLUPRORS |
| NCBI Protein Information: | bifunctional glutamate/proline--tRNA ligase |
| UniProt Protein Name: | Bifunctional glutamate/proline--tRNA ligase |
| UniProt Synonym Protein Names: | Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetaseIncluding the following 2 domains:Glutamate--tRNA ligase (EC:6.1.1.17)Alternative name(s):Glutamyl-tRNA synthetase; GluRS |
| UniProt Gene Name: | EPRS |
| UniProt Entry Name: | SYEP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EPRS antibody (CAB15245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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