Anti-ENTPD4 Antibody (CAB16469)
- SKU:
- CAB16469
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ENTPD4 Antibody |
| Antibody SKU: | CAB16469 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human ENTPD4 (NP_001122402.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse heart, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human ENTPD4 (NP_001122402.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTPD MPYL DPCL PLDI KDEI QQNG QTIY LRGT GDFD LCRE TIQP FMNK TNET QTSL NGVY QPPI HFQN SEFY GFSE FYYC TEDV LRMG GDYN AAKF TKAA KDYC ATKW SILR ERFD RGLY A |
| Gene ID: | 9583 |
| Uniprot: | Q9Y227 |
| Cellular Location: | Cytoplasmic vesicle, Golgi apparatus membrane, Multi-pass membrane protein, autophagosome membrane |
| Calculated MW: | 69kDa/70kDa |
| Observed MW: | 80kDa |
| Synonyms: | ENTPD4, LALP70, LAP70, LYSAL1, NTPDase-4, UDPase |
| Background: | This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. |
| UniProt Protein Function: | UDPase: an ectonucleoside triphosphate diphosphohydrolase. Hydrolyzes preferentially nucleoside 5'-diphosphates; nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP; AMP, ADP, ATP and UMP are not substrates. Two splice variant isoforms have been described. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP |
| UniProt Protein Details: | Protein type:EC 3.6.1.6; Nucleotide Metabolism - pyrimidine; Hydrolase; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Membrane protein, integral Chromosomal Location of Human Ortholog: 8p21.3 Cellular Component: integral to Golgi membrane; intracellular membrane-bound organelle Molecular Function:uridine-diphosphatase activity Biological Process: UDP catabolic process |
| NCBI Summary: | This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q9Y227 |
| NCBI GenInfo Identifier: | 18203627 |
| NCBI Gene ID: | 9583 |
| NCBI Accession: | Q9Y227.1 |
| UniProt Secondary Accession: | Q9Y227,O15092, D3DSS3, |
| UniProt Related Accession: | Q9Y227 |
| Molecular Weight: | 69,421 Da |
| NCBI Full Name: | Ectonucleoside triphosphate diphosphohydrolase 4 |
| NCBI Synonym Full Names: | ectonucleoside triphosphate diphosphohydrolase 4 |
| NCBI Official Symbol: | ENTPD4 |
| NCBI Official Synonym Symbols: | LAP70; LALP70; LYSAL1; UDPase; NTPDase-4 |
| NCBI Protein Information: | ectonucleoside triphosphate diphosphohydrolase 4 |
| UniProt Protein Name: | Ectonucleoside triphosphate diphosphohydrolase 4 |
| UniProt Synonym Protein Names: | Lysosomal apyrase-like protein of 70 kDa; Uridine-diphosphatase; UDPase |
| Protein Family: | Ectonucleoside triphosphate diphosphohydrolase |
| UniProt Gene Name: | ENTPD4 |
| UniProt Entry Name: | ENTP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ENTPD4 antibody (CAB16469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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