Anti-ENPP2 Antibody (CAB2522)

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SKU:
CAB2522
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-ENPP2 Antibody
Antibody SKU:CAB2522
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 564-863 of human ENPP2 (NP_001035181.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HepG2, A-549, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 564-863 of human ENPP2 (NP_001035181.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DLGC TCDD KVEP KNKL DELN KRLH TKGS TEER HLLY GRPA VLYR TRYD ILYH TDFE SGYS EIFL MPLW TSYT VSKQ AEVS SVPD HLTS CVRP DVRV SPSF SQNC LAYK NDKQ MSYG FLFP PYLS SSPE AKYD AFLV TNMV PMYP AFKR VWNY FQRV LVKK YASE RNGV NVIS GPIF DYDY DGLH DTED KIKQ YVEG SSIP VPTH YYSI ITSC LDFT QPAD KCDG PLSV SSFI LPHR PDNE ESCN SSED ESKW VEEL MKMH TARV RDIE HLTS LDFF RKTS RSYP EILT LKTY LHTY ESEI
Gene ID:5168
Uniprot:Q13822
Cellular Location:Secreted
Calculated MW:98kDa/101kDa/105kDa
Observed MW:110-115kDa
Synonyms:ENPP2, ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2
Background:The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - ether lipid; Phosphodiesterase; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.1.4.39

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function:nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; polysaccharide binding; lysophospholipase activity

Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; cell motility; chemotaxis; regulation of angiogenesis; phosphate metabolic process; regulation of cell migration

NCBI Summary:The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]
UniProt Code:Q13822
NCBI GenInfo Identifier:290457674
NCBI Gene ID:5168
NCBI Accession:Q13822.3
UniProt Related Accession:Q13822
Molecular Weight:
NCBI Full Name:Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
NCBI Synonym Full Names:ectonucleotide pyrophosphatase/phosphodiesterase 2
NCBI Official Symbol:ENPP2  
NCBI Official Synonym Symbols:ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA  
NCBI Protein Information:ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Protein Name:Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Synonym Protein Names:Autotaxin; Extracellular lysophospholipase D; LysoPLD
UniProt Gene Name:ENPP2  
UniProt Entry Name:ENPP2_HUMAN
Anti-ENPP2 Antibody (CAB2522)Western blot analysis of extracts of various cell lines, using ENPP2 antibody (CAB2522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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