Anti-eNOS Antibody (CAB1548)

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SKU:
CAB1548
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-eNOS Antibody
Antibody SKU:CAB1548
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human eNOS (NP_000594.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, LO2, HT-1080, K-562, Mouse liver, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human eNOS (NP_000594.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGNL KSVA QEPG PPCG LGLG LGLG LCGK QGPA TPAP EPSR APAS LLPP APEH SPPS SPLT QPPE GPKF PRVK NWEV GSIT YDTL SAQA QQDG PCTP RRCL GSLV FPRK LQGR PSPG PPAP
Gene ID:4846
Uniprot:P29474
Cellular Location:Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton
Calculated MW:67kDa/68kDa/133kDa
Observed MW:140kDa
Synonyms:NOS3, ECNOS, eNOS
Background:Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
UniProt Protein Function:eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function:actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding

Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic

NCBI Summary:Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Code:P29474
NCBI GenInfo Identifier:266648
NCBI Gene ID:4846
NCBI Accession:P29474.3
UniProt Secondary Accession:P29474,Q13662, Q14251, Q14434, Q548C1, Q6GSL5, Q9UDC6 A0S0A7, A0S0A8, A8KA63, B2RCQ1, E9PFR2,
UniProt Related Accession:P29474
Molecular Weight:
NCBI Full Name:Nitric oxide synthase, endothelial
NCBI Synonym Full Names:nitric oxide synthase 3
NCBI Official Symbol:NOS3  
NCBI Official Synonym Symbols:eNOS; ECNOS  
NCBI Protein Information:nitric oxide synthase, endothelial
UniProt Protein Name:Nitric oxide synthase, endothelial
UniProt Synonym Protein Names:Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Protein Family:Nitric oxide synthase
UniProt Gene Name:NOS3  
UniProt Entry Name:NOS3_HUMAN
Anti-eNOS Antibody (CAB1548)Western blot analysis of extracts of various cell lines, using eNOS antibody (CAB1548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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