Anti-EMD Antibody (CAB13925)
- SKU:
- CAB13925
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-EMD Antibody |
| Antibody SKU: | CAB13925 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EMD (NP_000108.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Jurkat, BxPC-3, U-251MG, Y79 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EMD (NP_000108.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDNY ADLS DTEL TTLL RRYN IPHG PVVG STRR LYEK KIFE YETQ RRRL SPPS SSAA SSYS FSDL NSTR GDAD MYDL PKKE DALL YQSK GYND DYYE ESYF TTRT YGEP ESAG PSRA VRQS VTSF PDAD AFHH QVHD DDLL SSSE EECK DRER PMYG RDSA YQSI THYR PVSA SRSS LDLS YYPT SSST SFMS SSSS SSSW LTRR AIRP ENRA PGAG LGQD |
| Gene ID: | 2010 |
| Uniprot: | P50402 |
| Cellular Location: | Nucleoplasmic side, Nucleus inner membrane, Nucleus outer membrane, Single-pass membrane protein |
| Calculated MW: | 28kDa |
| Observed MW: | 35kDa |
| Synonyms: | EMD, EDMD, LEMD5, STA, emerin |
| Background: | Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. |
| UniProt Protein Function: | emerin: a single pass membrane protein that spans the inner nuclear membrane. A serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It interacts with lamins A and C, and mediates membrane anchorage to the cytoskeleton. Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through an exportin 1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Colocalized with BAF at the central region of the assembling nuclear rim, near spindle-attachment sites. Hyper-phosphorylated on tyrosine in cells overexpressing HER2. Directly phosphorylated by Src and Abl. Phosphorylated by Src at Y59, Y74 and Y95. May function as a downstream effector and signal integrator for tyrosine kinase signaling pathways at the nuclear envelope. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: microtubule; nuclear outer membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear inner membrane; nuclear envelope Molecular Function:protein binding; beta-tubulin binding; actin binding Biological Process: positive regulation of protein export from nucleus; mitotic nuclear envelope reassembly; muscle development; muscle contraction; negative regulation of fibroblast proliferation; mitotic nuclear envelope disassembly; mitotic cell cycle Disease: Emery-dreifuss Muscular Dystrophy 1, X-linked |
| NCBI Summary: | Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50402 |
| NCBI GenInfo Identifier: | 1706639 |
| NCBI Gene ID: | 2010 |
| NCBI Accession: | P50402.1 |
| UniProt Secondary Accession: | P50402,Q6FI02, |
| UniProt Related Accession: | P50402 |
| Molecular Weight: | |
| NCBI Full Name: | Emerin |
| NCBI Synonym Full Names: | emerin |
| NCBI Official Symbol: | EMD |
| NCBI Official Synonym Symbols: | STA; EDMD; LEMD5 |
| NCBI Protein Information: | emerin; LEM domain containing 5 |
| UniProt Protein Name: | Emerin |
| Protein Family: | Emerin |
| UniProt Gene Name: | EMD |
| UniProt Entry Name: | EMD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EMD antibody (CAB13925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using EMD antibody (CAB13925) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterine cancer using EMD antibody (CAB13925) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using EMD antibody (CAB13925) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of U-2OS cells using EMD antibody (CAB13925) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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