Anti-ELSPBP1 Antibody (CAB15882)
- SKU:
- CAB15882
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ELSPBP1 Antibody |
| Antibody SKU: | CAB15882 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QQWK FCET NEYG GNSL RKPC IFPS IYRN NVVS DCME DESN KLWC PTTE NMDK DGKW SFCA DTRI SALV PGFP CHFP FNYK NKNY FNCT NEGS KENL VWCA TSYN YDQD HTWV YC |
| Gene ID: | 64100 |
| Uniprot: | Q96BH3 |
| Cellular Location: | Secreted |
| Calculated MW: | 26kDa |
| Observed MW: | 26kDa |
| Synonyms: | ELSPBP1, E12, EDDM12, EL149, HE12 |
| Background: | The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. |
| UniProt Protein Function: | ELSPBP1: Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity. Belongs to the seminal plasma protein family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19q13.33 Cellular Component: extracellular region Biological Process: single fertilization |
| NCBI Summary: | The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96BH3 |
| NCBI GenInfo Identifier: | 296434494 |
| NCBI Gene ID: | 64100 |
| NCBI Accession: | Q96BH3.2 |
| UniProt Related Accession: | Q96BH3 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | Epididymal sperm-binding protein 1 |
| NCBI Synonym Full Names: | epididymal sperm binding protein 1 |
| NCBI Official Symbol: | ELSPBP1 |
| NCBI Official Synonym Symbols: | E12; HE12; EL149; EDDM12 |
| NCBI Protein Information: | epididymal sperm-binding protein 1 |
| UniProt Protein Name: | Epididymal sperm-binding protein 1 |
| UniProt Synonym Protein Names: | Epididymal secretory protein 12; hE12 |
| Protein Family: | Epididymal sperm-binding protein |
| UniProt Gene Name: | ELSPBP1 |
| UniProt Entry Name: | ESPB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody (CAB15882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of rat testis using ELSPBP1 antibody (CAB15882) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse testis using ELSPBP1 antibody (CAB15882) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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