Anti-ELOVL4 항체 (CAB3639)

Antibody Name:	Anti-ELOVL4 Antibody
Antibody SKU:	CAB3639
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELOVL4 (NP_073563.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, OVCAR3, A-431, HeLa

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELOVL4 (NP_073563.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGLL DSEP GSVL NVVS TALN DTVE FYRW TWSI ADKR VENW PLMQ SPWP TLSI STLY LLFV WLGP KWMK DREP FQMR LVLI IYNF GMVL LNLF IFRE LFMG
Gene ID:	6785
Uniprot:	Q9GZR5
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	36kDa
Observed MW:	37kDa

Synonyms:

ELOVL4, ADMD, CT118, ISQMR, SCA34, STGD2, STGD3

Background:

This gene encodes a membrane-bound protein which is a member of the ELO family, proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina, mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD), also referred to as autosomal dominant atrophic macular degeneration.

UniProt Protein Function:	ELOVL4: Condensing enzyme that elongates saturated and monounsaturated very long chain fatty acids (VLCFAs). Elongates C24:0 and C26:0 acyl-CoAs. Seems to represent a photoreceptor- specific component of the fatty acid elongation system residing on the endoplasmic reticulum. May be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. May play a critical role in early brain and skin development. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). STGD is one of the most frequent causes of macular degeneration in childhood. It is characterized by macular dystrophy with juvenile-onset, rapidly progressive course, alterations of the peripheral retina, and subretinal deposition of lipofuscin-like material. STGD3 inheritance is autosomal dominant. Defects in ELOVL4 are the cause of ichthyosis, spastic quadriplegia, and mental retardation (ISQMR). A severe autosomal recessive disorder characterized by ichthyosis apparent from birth, profound psychomotor retardation with essentially no development, spastic quadriplegia, and seizures. Belongs to the ELO family.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA); Membrane protein, multi-pass; Endoplasmic reticulum; EC 2.3.1.199; Membrane protein, integral Chromosomal Location of Human Ortholog: 6q14 Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane Molecular Function:transferase activity; protein binding; G-protein coupled photoreceptor activity Biological Process: very-long-chain fatty acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid elongation, saturated fatty acid; fatty acid biosynthetic process; detection of visible light Disease: Spinocerebellar Ataxia 34
NCBI Summary:	This gene encodes a membrane-bound protein which is a member of the ELO family, proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina, mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD), also referred to as autosomal dominant atrophic macular degeneration. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9GZR5
NCBI GenInfo Identifier:	20137966
NCBI Gene ID:	6785
NCBI Accession:	Q9GZR5.1
UniProt Secondary Accession:	Q9GZR5,Q5TCS2, Q86YJ1, Q9H139, B2R6B5,
UniProt Related Accession:	Q9GZR5
Molecular Weight:	37kDa
NCBI Full Name:	Elongation of very long chain fatty acids protein 4
NCBI Synonym Full Names:	ELOVL fatty acid elongase 4
NCBI Official Symbol:	ELOVL4
NCBI Official Synonym Symbols:	ADMD; CT118; ISQMR; SCA34; STGD2; STGD3
NCBI Protein Information:	elongation of very long chain fatty acids protein 4; ELOVL FA elongase 4; cancer/testis antigen 118; 3-keto acyl-CoA synthase ELOVL4; very-long-chain 3-oxoacyl-CoA synthase 4; elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
UniProt Protein Name:	Elongation of very long chain fatty acids protein 4
UniProt Synonym Protein Names:	3-keto acyl-CoA synthase ELOVL4; ELOVL fatty acid elongase 4; ELOVL FA elongase 4; Very-long-chain 3-oxoacyl-CoA synthase 4
Protein Family:	Elongation of very long chain fatty acids protein
UniProt Gene Name:	ELOVL4
UniProt Entry Name:	ELOV4_HUMAN

	Western blot analysis of extracts of various cell lines, using ELOVL4 antibody (CAB3639) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using ELOVL4 Rabbit pAb (CAB3639) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using ELOVL4 Rabbit pAb (CAB3639) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ELOVL4 Antibody (PACO00756)

ELOVL4 Antibody (PACO09080)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)