Anti-ELF3 Antibody (CAB6371)
- SKU:
- CAB6371
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ELF3 Antibody |
| Antibody SKU: | CAB6371 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ELF3 (NP_004424.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | HT-29, SW480, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ELF3 (NP_004424.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAT CEIS NIFS NYFS AMYS SEDS TLAS VPPA ATFG ADDL VLTL SNPQ MSLE GTEK ASWL GEQP QFWS KTQV LDWI SYQV EKNK YDAS AIDF SRCD MDGA TLCN CALE ELRL VFGP LGDQ LHAQ LRDL TSSS SDEL SWII ELLE KDGM AFQE ALDP GPFD QGSP FAQE LLDD GQQA SPYH PGSC GAGA PSPG SSDV STAG TGAS RSSH SSDS GGSD VDLD PTDG KLFP SDGF RDCK KGDP KHGK RKRG RPRK LSKE YWDC LEGK KSKH APRG THLW EFIR |
| Gene ID: | 1999 |
| Uniprot: | P78545 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 39kDa/41kDa |
| Observed MW: | 42kDa |
| Synonyms: | ELF3, EPR-1, ERT, ESE-1, ESX |
| Background: |
| UniProt Protein Function: | ELF3: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; Motility/polarity/chemotaxis; DNA-binding Chromosomal Location of Human Ortholog: 1q32.2 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; mammary gland involution; epidermis development; extracellular matrix organization and biogenesis; blastocyst development; epithelial cell differentiation; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inflammatory response |
| UniProt Code: | P78545 |
| NCBI GenInfo Identifier: | 74739735 |
| NCBI Gene ID: | 1999 |
| NCBI Accession: | P78545.1 |
| UniProt Secondary Accession: | P78545,Q6IAP8, Q7RU03, Q7Z3X2, Q8NFG2, Q99718, B2R5Q6 |
| UniProt Related Accession: | P78545 |
| Molecular Weight: | |
| NCBI Full Name: | ETS-related transcription factor Elf-3 |
| NCBI Synonym Full Names: | E74-like factor 3 (ets domain transcription factor, epithelial-specific ) |
| NCBI Official Symbol: | ELF3 |
| NCBI Official Synonym Symbols: | ERT; ESX; EPR-1; ESE-1 |
| NCBI Protein Information: | ETS-related transcription factor Elf-3; ELF3; epithelial-restricted with serine box; epithelium-restricted Ets protein ESX; epithelium-specific Ets transcription factor 1; E74-like factor 3 (ets domain transcription factor); ets domain transcription factor, serine box (epithelial-specific); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific) |
| UniProt Protein Name: | ETS-related transcription factor Elf-3 |
| UniProt Synonym Protein Names: | E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1 |
| Protein Family: | ELF3-like protein |
| UniProt Gene Name: | ELF3 |
| UniProt Entry Name: | ELF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ELF3 antibody (CAB6371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using ELF3 antibody (CAB6371) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of A549 cells using ELF3 antibody (CAB6371). Blue: DAPI for nuclear staining. |
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