Anti-EIF4E2 Antibody (CAB4305)
- SKU:
- CAB4305
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EIF4E2 Antibody |
| Antibody SKU: | CAB4305 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF4E2 (NP_004837.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, HL-60, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF4E2 (NP_004837.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNNK FDAL KDDD SGDH DQNE ENST QKDG EKEK TERD KNQS SSKR KAVV PGPA EHPL QYNY TFWY SRRT PGRP TSSQ SYEQ NIKQ IGTF ASVE QFWR FYSH MVRP GDLT GHSD FHLF KEGI KPMW EDDA NKNG GKWI IRLR KGLA SRCW ENLI LAML GEQF MVGE EICG AVVS VRFQ EDII SIWN KTAS DQAT TARI RDTL RRVL NLPP NTIM EYKT HTDS IKMP GRLG PQRL LFQN LWKP |
| Gene ID: | 9470 |
| Uniprot: | O60573 |
| Cellular Location: | |
| Calculated MW: | 27kDa/28kDa |
| Observed MW: | 28kDa |
| Synonyms: | EIF4E2, 4E-LP, 4EHP, EIF4EL3, IF4e, h4EHP |
| Background: |
| UniProt Protein Function: | EIF4E2: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners. EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G. Belongs to the eukaryotic initiation factor 4E family. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: mRNA cap complex; cytosol Molecular Function:protein binding; translation factor activity, nucleic acid binding; ubiquitin protein ligase binding; translation initiation factor activity; RNA cap binding Biological Process: in utero embryonic development; cytokine and chemokine mediated signaling pathway; negative regulation of translation; translational initiation |
| UniProt Code: | O60573 |
| NCBI GenInfo Identifier: | 29839256 |
| NCBI Gene ID: | 9470 |
| NCBI Accession: | O60573.1 |
| UniProt Secondary Accession: | O60573,O75349, B8ZZJ9, |
| UniProt Related Accession: | O60573 |
| Molecular Weight: | 245 |
| NCBI Full Name: | Eukaryotic translation initiation factor 4E type 2 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E family member 2 |
| NCBI Official Symbol: | EIF4E2 |
| NCBI Official Synonym Symbols: | 4EHP; IF4e; 4E-LP; EIF4EL3 |
| NCBI Protein Information: | eukaryotic translation initiation factor 4E type 2; eIF-4E type 2; eIF4E-like protein 4E-LP; eIF4E-like cap-binding protein; mRNA cap-binding protein type 3; eukaryotic translation initiation factor 4E-like 3; eukaryotic translation initiation factor 4E homologous protein |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4E type 2 |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3 |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4E2 |
| UniProt Entry Name: | IF4E2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF4E2 antibody (CAB4305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using EIF4E2 Rabbit pAb (CAB4305) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using EIF4E2 Rabbit pAb (CAB4305) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using EIF4E2 Rabbit pAb (CAB4305) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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