Anti-EIF4A1 Antibody (CAB5294)
- SKU:
- CAB5294
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-EIF4A1 Antibody |
| Antibody SKU: | CAB5294 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:50 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, MCF7, C6, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAS QDSR SRDN GPDG MEPE GVIE SNWN EIVD SFDD MNLS ESLL RGIY AYGF EKPS AIQQ RAIL PCIK GYDV IAQA QSGT GKTA TFAI SILQ QIEL DLKA TQAL VLAP TREL AQQI QKVV MALG DYMG ASCH ACIG GTNV RAEV QKLQ MEAP HIIV GTPG RVFD MLNR RYLS PKYI KMFV LDEA DEML SRGF KDQI YDIF QKLN SNTQ VVLL SATM PSDV LEVT KKFM RDPI RILV KKEE LTLE GIRQ FYIN VERE EWKL DTLC DLYE TLTI TQAV IFIN TRRK VDWL TEKM HARD FTVS AMHG DMDQ KERD VIMR EFRS GSSR VLIT TDLL ARGI DVQQ VSLV INYD LPTN RENY IHRI GRGG RFGR KGVA INMV TEED KRTL RDIE TFYN TSIE EMPL NVAD LI |
| Gene ID: | 1973 |
| Uniprot: | P60842 |
| Cellular Location: | |
| Calculated MW: | 39kDa/46kDa |
| Observed MW: | 45KDa |
| Synonyms: | EIF4A1, DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I |
| Background: |
| UniProt Protein Function: | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
| UniProt Code: | P60842 |
| NCBI GenInfo Identifier: | 46397463 |
| NCBI Gene ID: | 1973 |
| NCBI Accession: | P60842.1 |
| UniProt Secondary Accession: | P60842,P04765, Q5U018, Q61516, B2R6L8, D3DTP9, J3QLC4 |
| UniProt Related Accession: | P60842 |
| Molecular Weight: | 39,548 Da |
| NCBI Full Name: | Eukaryotic initiation factor 4A-I |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4A1 |
| NCBI Official Symbol: | EIF4A1 |
| NCBI Official Synonym Symbols: | DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I |
| NCBI Protein Information: | eukaryotic initiation factor 4A-I |
| UniProt Protein Name: | Eukaryotic initiation factor 4A-I |
| UniProt Synonym Protein Names: | ATP-dependent RNA helicase eIF4A-1 |
| Protein Family: | Eukaryotic initiation factor |
| UniProt Gene Name: | EIF4A1 |
| UniProt Entry Name: | IF4A1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (CAB5294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (CAB5294). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug EIF4A1 antibody (CAB5294). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (CAB5294) at a dilition of 1:1000. |
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