Anti-EIF3H Antibody (CAB7024)

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SKU:
CAB7024
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-EIF3H Antibody
Antibody SKU:CAB7024
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, HeLa, Jurkat, Mouse spleen, Mouse heart, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NCFP FPQH TEDD ADFD EVQY QMEM MRSL RHVN IDHL HVGW YQST YYGS FVTR ALLD SQFS YQHA IEES VVLI YDPI KTAQ GSLS LKAY RLTP KLME VCKE KDFS PEAL KKAN ITFE YMFE EVPI VIKN SHLI NVLM WELE KKSA VADK HELL SLAS SNHL GKNL QLLM DRVD EMSQ DIVK YNTY MRNT SKQQ QQKH QYQQ RRQQ ENMQ RQSR GEPP LPEE DLSK LFKP PQPP ARMD SLLI AGQI NTYC QNIK EFTA QNLG K
Gene ID:8667
Uniprot:O15372
Cellular Location:Cytoplasm
Calculated MW:39kDa
Observed MW:40kDa
Synonyms:EIF3H, EIF3S3, eIF3-gamma, eIF3-p40
Background:
UniProt Protein Function:eIF3S3: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination- dependent manner. Belongs to the eIF-3 subunit H family.
UniProt Protein Details:

Protein type:Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytosol

Molecular Function:protein binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation

UniProt Code:O15372
NCBI GenInfo Identifier:6685512
NCBI Gene ID:8667
NCBI Accession:O15372.1
UniProt Related Accession:O15372
Molecular Weight:352
NCBI Full Name:Eukaryotic translation initiation factor 3 subunit H
NCBI Synonym Full Names:eukaryotic translation initiation factor 3, subunit H
NCBI Official Symbol:EIF3H  
NCBI Official Synonym Symbols:EIF3S3; eIF3-p40; eIF3-gamma  
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit H; EIF3H; eIF-3-gamma; eIF3 p40 subunit; eukaryotic translation initiation factor 3 subunit 3; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); eukaryotic translation initiation factor
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit H
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gammaeIF3 p40 subunit
UniProt Gene Name:EIF3H  
UniProt Entry Name:EIF3H_HUMAN
Anti-EIF3H Antibody (CAB7024)Western blot analysis of extracts of various cell lines, using EIF3H antibody (CAB7024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EIF3H Antibody (CAB7024)Immunofluorescence analysis of U2OS cells using EIF3H antibody (CAB7024). Blue: DAPI for nuclear staining.
Anti-EIF3H Antibody (CAB7024)Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody (CAB7024). Western blot was performed from the immunoprecipitate using EIF3H antibody (CAB7024) at a dilition of 1:1000.

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