Anti-EIF3G Antibody (CAB4240)
- SKU:
- CAB4240
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EIF3G Antibody |
| Antibody SKU: | CAB4240 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, Jurkat, HeLa, NIH/3T3, HL-60, K-562, Mouse Kidney, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPTG DFDS KPSW ADQV EEEG EDDK CVTS ELLK GIPL ATGD TSPE PELL PGAP LPPP KEVI NGNI KTVT EYKI DEDG KKFK IVRT FRIE TRKA SKAV ARRK NWKK FGNS EFDP PGPN VATT TVSD DVSM TFIT SKED LNCQ EEED PMNK LKGQ KIVS CRIC KGDH WTTR CPYK DTLG PMQK ELAE QLGL STGE KEKL PGEL EPVQ ATQN KTGK YVPP SLRD GASR RGES MQPN RRAD DNAT IRVT NLSE DTRE TDLQ ELFR PFGS ISRI YLAK DKTT GQSK GFAF ISFH RRED AARA IAGV SGFG YDHL ILNV EWAK PSTN |
| Gene ID: | 8666 |
| Uniprot: | O75821 |
| Cellular Location: | Cytoplasm, Nucleus, perinuclear region |
| Calculated MW: | 35kDa |
| Observed MW: | 42kDa |
| Synonyms: | EIF3G, EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44 |
| Background: |
| UniProt Protein Function: | eIF3-delta: eukaryotic translation initiation factor 3 subunit 4. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. This subunit binds to the 18S rRNA. eIF-3 is composed of at least 12 different subunits. Contains 1 RNA recognition motif (RRM) domain. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation; RNA-binding Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: eukaryotic translation initiation factor 3 complex; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; translation initiation factor activity; nucleotide binding Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation |
| UniProt Code: | O75821 |
| NCBI GenInfo Identifier: | 23503064 |
| NCBI Gene ID: | 8666 |
| NCBI Accession: | O75821.2 |
| UniProt Secondary Accession: | O75821,O14801, Q969U5, |
| UniProt Related Accession: | O75821 |
| Molecular Weight: | 35,611 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit G |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3, subunit G |
| NCBI Official Symbol: | EIF3G |
| NCBI Official Synonym Symbols: | EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit G; eIF3 p42; eIF3 p44; eIF-3-delta; eIF-3 RNA-binding subunit; eukaryotic translation initiation factor 3 subunit 4; eukaryotic translation initiation factor 3 subunit p42; eukaryotic translation initiation factor 3 RNA-binding subunit; eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit G |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 RNA-binding subunit; eIF-3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44 |
| UniProt Gene Name: | EIF3G |
| UniProt Entry Name: | EIF3G_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3G antibody (CAB4240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (CAB4240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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