Anti-EIF3E Antibody (CAB5447)
- SKU:
- CAB5447
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EIF3E Antibody |
| Antibody SKU: | CAB5447 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human EIF3E (NP_001559.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, K-562, HepG2, Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human EIF3E (NP_001559.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEY DLTT RIAH FLDR HLVF PLLE FLSV KEIY NEKE LLQG KLDL LSDT NMVD FAMD VYKN LYSD DIPH ALRE KRTT VVAQ LKQL QAET EPIV KMFE DPET TRQM QSTR DGRM LFDY LADK HGFR QEYL DTLY RYAK FQYE CGNY SGAA EYLY FFRV LVPA TDRN ALSS LWGK LASE ILMQ NWDA AMED LTRL KETI DNNS VSSP LQSL QQRT WLIH WSLF VFFN HPKG RDNI IDLF LYQP QYLN AIQT MCPH ILRY LTTA VITN KDVR KRRQ VLKD LVKV IQQE SYTY KDPI TEFV ECLY VNFD FDGA QKKL RECE SVLV NDFF LVAC LEDF IENA RLFI FETF CRIH QCIS INML ADKL NMTP EEAE RWIV NLIR NARL DAKI DSKL GHVV MGNN AVSP YQQV IEKT KSLS FRSQ MLAM NIEK KLNQ NSRS EAPN WATQ DSGF Y |
| Gene ID: | 3646 |
| Uniprot: | P60228 |
| Cellular Location: | Cytoplasm, Nucleus, PML body |
| Calculated MW: | 52kDa |
| Observed MW: | 52kDa |
| Synonyms: | EIF3E, EIF3-P48, EIF3S6, INT6, eIF3-p46 |
| Background: |
| UniProt Protein Function: | EIF3E: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 8q22-q23 Cellular Component: eukaryotic translation initiation factor 3 complex; nucleoplasm; PML body; membrane; cytoplasm; cytosol; chromatin; nucleus Molecular Function:protein binding; translation initiation factor activity; protein N-terminus binding Biological Process: negative regulation of translational initiation; cellular protein metabolic process; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation |
| UniProt Code: | P60228 |
| NCBI GenInfo Identifier: | 41019126 |
| NCBI Gene ID: | 3646 |
| NCBI Accession: | P60228.1 |
| UniProt Secondary Accession: | P60228,O43902, Q64058, Q64059, Q64252, Q6FG33, Q8WVK4 |
| UniProt Related Accession: | P60228 |
| Molecular Weight: | 52,221 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit E |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3, subunit E |
| NCBI Official Symbol: | EIF3E |
| NCBI Official Synonym Symbols: | INT6; EIF3S6; EIF3-P48; eIF3-p46 |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit E; eIF-3 p48; mammary tumor-associated protein INT6; viral integration site protein INT-6 homolog; eukaryotic translation initiation factor 3 subunit 6; murine mammary tumor integration site 6 (oncogene homolog); eukaryotic translation initiation factor 3, subunit 6 48kDa |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit E |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48 |
| UniProt Gene Name: | EIF3E |
| UniProt Entry Name: | EIF3E_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3E antibody (CAB5447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using EIF3E antibody (CAB5447). |
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