Anti-EIF2B5 Antibody (CAB10263)
- SKU:
- CAB10263
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EIF2B5 Antibody |
| Antibody SKU: | CAB10263 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human EIF2B5 (NP_003898.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, HL-60, HT-1080, LO2, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human EIF2B5 (NP_003898.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TLPE GSVI SLHP PDAE EDED DGEF SDDS GADQ EKDK VKMK GYNP AEVG AAGK GYLW KAAG MNME EEEE LQQN LWGL KINM EEES ESES EQSM DSEE PDSR GGSP QMDD IKVF QNEV LGTL QRGK EENI SCDN LVLE INSL KYAY NISL KEVM QVLS HVVL EFPL QQMD SPLD SSRY CALL LPLL KAWS PVFR NYIK RAAD HLEA LAAI EDFF LEHE ALGI SMAK VLMA FYQL EILA EETI LSWF SQRD TTDK GQQL RKNQ QLQR FIQW LKEA EEES SEDD |
| Gene ID: | 8893 |
| Uniprot: | Q13144 |
| Cellular Location: | |
| Calculated MW: | 80kDa |
| Observed MW: | 85kDa |
| Synonyms: | EIF2B5, CACH, CLE, EIF-2B, EIF2Bepsilon, LVWM |
| Background: | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. |
| UniProt Protein Function: | eIF2B-epsilon: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 3q27.1 Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 2B complex; nucleus Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; translation initiation factor activity; translation initiation factor binding Biological Process: astrocyte development; astrocyte differentiation; myelination; negative regulation of translation initiation in response to stress; oligodendrocyte development; ovarian follicle development; positive regulation of translational initiation; response to glucose stimulus; response to heat; response to peptide hormone stimulus; translational initiation Disease: Leukoencephalopathy With Vanishing White Matter |
| NCBI Summary: | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009] |
| UniProt Code: | Q13144 |
| NCBI GenInfo Identifier: | 160359049 |
| NCBI Gene ID: | 8893 |
| NCBI Accession: | Q13144.3 |
| UniProt Secondary Accession: | Q13144,Q541Z1, Q96D04, |
| UniProt Related Accession: | Q13144 |
| Molecular Weight: | 80kDa |
| NCBI Full Name: | Translation initiation factor eIF-2B subunit epsilon |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2B subunit epsilon |
| NCBI Official Symbol: | EIF2B5 |
| NCBI Official Synonym Symbols: | CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon |
| NCBI Protein Information: | translation initiation factor eIF-2B subunit epsilon |
| UniProt Protein Name: | Translation initiation factor eIF-2B subunit epsilon |
| UniProt Synonym Protein Names: | eIF-2B GDP-GTP exchange factor subunit epsilon |
| Protein Family: | Translation initiation factor |
| UniProt Gene Name: | EIF2B5 |
 | Western blot analysis of extracts of various cell lines, using EIF2B5 antibody (CAB10263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using EIF2B5 Rabbit pAb (CAB10263) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug EIF2B5 antibody ( A10263). Western blot was performed from the immunoprecipitate using EIF2B5 antibody ( A10263) at a dilition of 1:1000. |
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