Anti-EIF1AY Antibody (CAB4270)
- SKU:
- CAB4270
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EIF1AY Antibody |
| Antibody SKU: | CAB4270 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human EIF1AY (NP_004672.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, MCF7, Raji, Jurkat, U-251MG, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human EIF1AY (NP_004672.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPKN KGKG GKNR RRGK NENE SEKR ELVF KEDG QEYA QVIK MLGN GRLE ALCF DGVK RLCH IRGK LRKK VWIN TSDI ILVG LRDY QDNK ADVI LKYN ADEA RSLK AYGE LPEH AKIN ETDT FGPG DDDE IQFD DIGD DDED IDDI |
| Gene ID: | 9086 |
| Uniprot: | O14602 |
| Cellular Location: | |
| Calculated MW: | 16kDa |
| Observed MW: | 18kDa |
| Synonyms: | EIF1AY, eIF-4C, Y-linked |
| Background: | This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | EIF1AY: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: Yq11.223 Molecular Function:protein binding; translation initiation factor activity Biological Process: translational initiation |
| NCBI Summary: | This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O14602 |
| NCBI GenInfo Identifier: | 25014072 |
| NCBI Gene ID: | 9086 |
| NCBI Accession: | O14602.4 |
| UniProt Secondary Accession: | O14602,Q9BS77, |
| UniProt Related Accession: | O14602 |
| Molecular Weight: | 16,442 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 1A, Y-chromosomal |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 1A, Y-linked |
| NCBI Official Symbol: | EIF1AY |
| NCBI Official Synonym Symbols: | eIF-4C |
| NCBI Protein Information: | eukaryotic translation initiation factor 1A, Y-chromosomal; eIF-1A Y isoform; eukaryotic translation initiation factor 1A, Y chromosome; eukaryotic translation initiation factor 4C |
| UniProt Protein Name: | Eukaryotic translation initiation factor 1A, Y-chromosomal |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 4C; eIF-4C |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF1AY |
| UniProt Entry Name: | IF1AY_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF1AY antibody (CAB4270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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