Anti-EHMT2 Antibody (CAB1247)[KO Validated]

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SKU:
CAB1247
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-EHMT2 Antibody [KO Validated]
Antibody SKU:CAB1247
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-937, 293T, Jurkat, MCF7, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VGNR AIRT EKII CRDV ARGY ENVP IPCV NGVD GEPC PEDY KYIS ENCE TSTM NIDR NITH LQHC TCVD DCSS SNCL CGQL SIRC WYDK DGRL LQEF NKIE PPLI FECN QACS CWRN CKNR VVQS GIKV RLQL YRTA KMGW GVRA LQTI PQGT FICE YVGE LISD AEAD VRED DSYL FDLD NKDG EVYC IDAR YYGN ISRF INHL CDPN IIPV RVFM LHQD LRFP RIAF FSSR DIRT GEEL GFDY GDRF WDIK SKYF TCQC GSEK CKHS AEAI ALEQ SRLA RLDP HPEL LPEL GSLP PVNT
Gene ID:10919
Uniprot:Q96KQ7
Cellular Location:Chromosome, Nucleus
Calculated MW:19kDa/129kDa/132kDa
Observed MW:132kDa
Synonyms:EHMT2, BAT8, C6orf30, G9A, GAT8, KMT1C, NG36
Background:This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:EHMT2 iso4: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus

Molecular Function:protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA replication; histone H3-K9 methylation

NCBI Summary:This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:Q96KQ7
NCBI GenInfo Identifier:116241348
NCBI Gene ID:10919
NCBI Accession:Q96KQ7.3
UniProt Secondary Accession:Q96KQ7,Q14349, Q5JP83, Q5JQ92, Q5JQA1, Q5JQG3, Q6PK06 Q96MH5, Q96QD0, Q9UQL8, Q9Y331, B0UZY2,
UniProt Related Accession:Q96KQ7
Molecular Weight:1210
NCBI Full Name:Histone-lysine N-methyltransferase EHMT2
NCBI Synonym Full Names:euchromatic histone-lysine N-methyltransferase 2
NCBI Official Symbol:EHMT2  
NCBI Official Synonym Symbols:G9A; BAT8; GAT8; NG36; KMT1C; C6orf30  
NCBI Protein Information:histone-lysine N-methyltransferase EHMT2; H3-K9-HMTase 3; G9A histone methyltransferase; HLA-B associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase
UniProt Protein Name:Histone-lysine N-methyltransferase EHMT2
UniProt Synonym Protein Names:Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a
UniProt Gene Name:EHMT2  
UniProt Entry Name:EHMT2_HUMAN
Anti-EHMT2 Antibody [KO Validated] (CAB1247)Western blot analysis of extracts of various cell lines, using EHMT2 antibody (CAB1247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-EHMT2 Antibody [KO Validated] (CAB1247)Western blot analysis of extracts from normal (control) and EHMT2 Rabbit pAb knockout (KO) 293T cells, using EHMT2 Rabbit pAb antibody (CAB1247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

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