Anti-ECI1 Antibody (CAB1211)
- SKU:
- CAB1211
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ECI1 Antibody |
| Antibody SKU: | CAB1211 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 63-302 of human ECI1 (NP_001910.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BxPC-3, HeLa, 22Rv1, Mouse heart, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 63-302 of human ECI1 (NP_001910.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KNPP VNSL SLEF LTEL VISL EKLE NDKS FRGV ILTS DRPG VFSA GLDL TEMC GRSP AHYA GYWK AVQE LWLR LYQS NLVL VSAI NGAC PAGG CLVA LTCD YRIL ADNP RYCI GLNE TQLG IIAP FWLK DTLE NTIG HRAA ERAL QLGL LFPP AEAL QVGI VDQV VPEE QVQS TALS AIAQ WMAI PDHA RQLT KAMM RKAT ASRL VTQR DADV QNFV SFIS KDSI QKSL QMYL ERLK EEKG |
| Gene ID: | 1632 |
| Uniprot: | P42126 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 30kDa/32kDa |
| Observed MW: | 34kDa |
| Synonyms: | ECI1, DCI |
| Background: | This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 5.3.3.8; Isomerase; Mitochondrial; Lipid Metabolism - fatty acid Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process |
| NCBI Summary: | This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010] |
| UniProt Code: | P42126 |
| NCBI GenInfo Identifier: | 1169204 |
| NCBI Gene ID: | 1632 |
| NCBI Accession: | P42126.1 |
| UniProt Related Accession: | P42126 |
| Molecular Weight: | |
| NCBI Full Name: | Enoyl-CoA delta isomerase 1, mitochondrial |
| NCBI Synonym Full Names: | enoyl-CoA delta isomerase 1 |
| NCBI Official Symbol: | ECI1 |
| NCBI Official Synonym Symbols: | DCI |
| NCBI Protein Information: | enoyl-CoA delta isomerase 1, mitochondrial |
| UniProt Protein Name: | Enoyl-CoA delta isomerase 1, mitochondrial |
| UniProt Synonym Protein Names: | 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase |
| UniProt Gene Name: | ECI1 |
| UniProt Entry Name: | ECI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ECI1 antibody (CAB1211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using ECI1 antibody (CAB1211). Blue: DAPI for nuclear staining. |
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