Anti-EARS2 Antibody (CAB14959)
- SKU:
- CAB14959
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-EARS2 Antibody |
| Antibody SKU: | CAB14959 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APSP TGFL HLGG LRTA LYNY IFAK KYQG SFIL RLED TDQT RVVP GAAE NIED MLEW AGIP PDES PRRG GPAG PYQQ SQRL ELYA QATE ALLK TGAA YPCF CSPQ RLEL LKKE ALRN HQTP RYDN RCRN MSQE QVAQ KLAK DPKP AIRF RLEQ VVPA FQDL VYGW NRHE VASV EGDP VIM |
| Gene ID: | 124454 |
| Uniprot: | Q5JPH6 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 58kDa/59kDa |
| Observed MW: | 59kDa |
| Synonyms: | EARS2, COXPD12, MSE1, gluRS |
| Background: | This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | EARS2: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Belongs to the class-I aminoacyl-tRNA synthetase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Mitochondrial; Translation; EC 6.1.1.17; Ligase Chromosomal Location of Human Ortholog: 16p12.2 Cellular Component: mitochondrion Molecular Function:glutamate-tRNA ligase activity; glutamate-tRNA(Gln) ligase activity; RNA binding Biological Process: glutamyl-tRNA aminoacylation Disease: Combined Oxidative Phosphorylation Deficiency 12 |
| NCBI Summary: | This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
| UniProt Code: | Q5JPH6 |
| NCBI GenInfo Identifier: | 117949790 |
| NCBI Gene ID: | 124454 |
| NCBI Accession: | Q5JPH6.2 |
| UniProt Secondary Accession: | Q5JPH6,Q86YH3, Q8TF31, B3KTT2, D3DWF1, |
| UniProt Related Accession: | Q5JPH6 |
| Molecular Weight: | 59,989 Da |
| NCBI Full Name: | Probable glutamate--tRNA ligase, mitochondrial |
| NCBI Synonym Full Names: | glutamyl-tRNA synthetase 2, mitochondrial |
| NCBI Official Symbol: | EARS2 |
| NCBI Official Synonym Symbols: | MSE1; gluRS; COXPD12 |
| NCBI Protein Information: | probable glutamate--tRNA ligase, mitochondrial |
| UniProt Protein Name: | Probable glutamate--tRNA ligase, mitochondrial |
| UniProt Synonym Protein Names: | Glutamyl-tRNA synthetase; GluRS |
| Protein Family: | Probable glutamate--tRNA ligase |
| UniProt Gene Name: | EARS2 |
| UniProt Entry Name: | SYEM_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using EARS2 antibody (CAB14959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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