Anti-E2F3 Antibody (CAB8811)
- SKU:
- CAB8811
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-E2F3 Antibody |
| Antibody SKU: | CAB8811 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SIES LQIH LAST QGPI EVYL CPEE TETH SPMK TNNQ DHNG NIPK PASK DLAS TNSG HSDC SVSM GNLS PLAS PANL LQQT EDQI PSNL EGPF VNLL PPLL Q |
| Gene ID: | 1871 |
| Uniprot: | O00716 |
| Cellular Location: | Nucleus |
| Calculated MW: | 37kDa/49kDa |
| Observed MW: | 53kDa |
| Synonyms: | E2F3, E2F-3 |
| Background: | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | E2F3: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding; Cell cycle regulation Chromosomal Location of Human Ortholog: 6p22 Cellular Component: nucleoplasm; Golgi apparatus; transcription factor complex; intracellular membrane-bound organelle; cytoplasm; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; mitotic cell cycle |
| NCBI Summary: | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
| UniProt Code: | O00716 |
| NCBI GenInfo Identifier: | 2811006 |
| NCBI Gene ID: | 1871 |
| NCBI Accession: | O00716.1 |
| UniProt Related Accession: | O00716 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor E2F3 |
| NCBI Synonym Full Names: | E2F transcription factor 3 |
| NCBI Official Symbol: | E2F3 |
| NCBI Official Synonym Symbols: | E2F-3 |
| NCBI Protein Information: | transcription factor E2F3 |
| UniProt Protein Name: | Transcription factor E2F3 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | E2F3 |
| UniProt Entry Name: | E2F3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using E2F3 antibody (CAB8811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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