Anti-DYNLL1 항체 (CAB14496)

Antibody Name:	Anti-DYNLL1 Antibody
Antibody SKU:	CAB14496
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-89 of human DYNLL1 (NP_003737.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HeLa, A-549, 293T, NIH/3T3, Rat spinal cord

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-89 of human DYNLL1 (NP_003737.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MCDR KAVI KNAD MSEE MQQD SVEC ATQA LEKY NIEK DIAA HIKK EFDK KYNP TWHC IVGR NFGS YVTH ETKH FIYF YLGQ VAIL LFKS G
Gene ID:	8655
Uniprot:	P63167
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, cytoskeleton
Calculated MW:	10kDa
Observed MW:	12kDa

Synonyms:

DYNLL1, DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1

Background:

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1, 200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.

UniProt Protein Function:	LC8: a highly conserved cytoplasmic protein of the dynein light chain family. Cytoplasmic dyneins are large enzyme complexes. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain. Interacts with and inhibits the activity of neuronal nitric oxide synthase (nNOS). A highly conserved protein, showing 92% amino acid similarity with the nematode homolog. Binding of this protein destabilizes the nNOS dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity.
UniProt Protein Details:	Protein type:Microtubule-binding; Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 12q24.23 Cellular Component: kinetochore; signalosome; microtubule; centrosome; membrane; cytoplasmic dynein complex; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol Molecular Function:protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; protein homodimerization activity; nitric-oxide synthase regulator activity; motor activity Biological Process: anatomical structure morphogenesis; viral reproduction; transcription, DNA-dependent; apoptosis; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; female gamete generation; microtubule-based process; substantia nigra development; regulation of transcription, DNA-dependent; transport; regulation of catalytic activity; negative regulation of phosphorylation; mitotic cell cycle; actin cytoskeleton organization and biogenesis; G2/M transition of mitotic cell cycle
NCBI Summary:	Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P63167
NCBI GenInfo Identifier:	52783578
NCBI Gene ID:	8655
NCBI Accession:	P63167.1
UniProt Secondary Accession:	P63167,Q15701,
UniProt Related Accession:	P63167
Molecular Weight:	89
NCBI Full Name:	Dynein light chain 1, cytoplasmic
NCBI Synonym Full Names:	dynein, light chain, LC8-type 1
NCBI Official Symbol:	DYNLL1
NCBI Official Synonym Symbols:	LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
NCBI Protein Information:	dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; cytoplasmic dynein light polypeptide; dynein, cytoplasmic, light polypeptide 1; protein inhibitor of neuronal nitric oxide synthase
UniProt Protein Name:	Dynein light chain 1, cytoplasmic
UniProt Synonym Protein Names:	8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN
UniProt Gene Name:	DYNLL1
UniProt Entry Name:	DYL1_HUMAN

	Western blot analysis of extracts of various cell lines, using DYNLL1 antibody (CAB14496) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat ovary using DYNLL1 Rabbit pAb (CAB14496) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using DYNLL1 Rabbit pAb (CAB14496) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using DYNLL1 Rabbit pAb (CAB14496) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using DYNLL1 Rabbit pAb (CAB14496) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-DYNLL1 Antibody (CAB5742)

Anti-DYNLL1 Antibody (CAB4353)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)